茵陈总黄酮的提取及富集工艺

Extraction and enrichment process of total flavonoids from Artemisia capillaris Thunb.

采用超声波辅助提取法对茵陈总黄酮的提取和富集工艺进行研究,用正交实验优化茵陈总黄酮的最优提取工艺为:在40℃时,以料液比1∶15(g/m L)的蒸馏水超声提取60 min.以6种大孔吸附树脂对茵陈总黄酮的富集作用进行研究,结果表明D101型树脂对黄酮的吸附和解吸附效果最好.进一步优化最佳富集工艺条件为:上样液质量浓度0.55 mg/m L,pH 4.04,体积流量1.5 BV/h,洗脱溶剂乙醇质量分数70%.

The extraction and enrichment of total flavonoids from Artemisia capillaris Thunb. were studied by ultrasonic as-sistant extraction. The optimal extraction process obtained by orthogonal experiment was extraction 60 min by ul-trasonic with distilled water at 40℃ and liquid ratio was 1 - 15（g/mL）. Six kinds of macroporous resin were used to study the enrichment of flavonoids, and the results showed that D101 resin was the most appropriate. Further optimizing the optimum conditions for the enrichment process, when the conditions were the sampling concentra-tion of 0.55 mg/mL, pH 4.04, volume flow rate of 1.5 BV/h, and ethanol concentration of 70 % , the purified flavonoids could be scientifically and reasonably separated.