副溶血性弧菌16S rRNA基因序列变异规律分析

Sequence Variation Regularity Analysis of 16S rRNA Gene of Vibrio parahaemolyticus

通过对副溶血性弧菌16S rRNA基因序列进行深度分析，研究其序列变异特点，为开发新的分型溯源靶点奠定基础。对111株分离自生鲜水产品的副溶血性弧菌16S rRNA基因测序，与GenBank中已有的副溶血性弧菌16S rRNA基因标准序列比对分析，对变异度比较大的序列建立了系统发育树，深入分析了基因的变异规律，并使用ERIC-PCR方法加以验证。不同副溶血性弧菌菌株16S rRNA基因变异主要集中在可变区V1和V2区，50-270 bp的区域内有38个碱基的相似度小于30%，103个碱基的相似度在30%-100%。另外，在V3区和V8区也各有1个碱基有比较明显的差异。副溶血性弧菌16S rRNA基因序列具有一定的变异性，该结果为副溶血性弧菌菌株分型溯源分析提供新的靶点奠定研究基础，同时为其微进化研究奠定理论基础。

In order to lay a foundation for the development of new targets for typing and tracing, we investigated the characteristics of the variations based on the analysis of 16S rRNA gene sequences of Vibrio parahaemolyticussequenced 16S rRNA gene of 111 strains of Vibrio parahaemolyticus which were isolated from fresh aquatic products, and aligned with standard sequence in GenBank. We also established phylogenetic tree of sequences which had a great variation,and verified the variation regularity of the genes by ERIC-PCR.The variability of different Vibrio parahaemolyticus of 16S rRNA genes was discovered. The variation mainly centralized in variable region V1 and V2.There were 38 base sites which similarity were less than 30% in the region of 50-270 bp,and 103 base sites wliich similarity ranged from 30% to 100%, In addition,there was one base wliich showed difference both at V3 and V8. The variability of different Vibrio par alum molytkus of 16S rRNA genes can lay a foundation for the development of new targets for typing and tracing of Vibrio parahaemolyticus and for the study of micro-evolution.