Desmin在结直肠癌组织中的表达及其临床意义

The Expression of Desmin in Human Colorectal Cancer and Its Clinical Significance

为探讨Desmin在结直肠癌组织中的表达情况及其临床意义，本研究使用组织芯片、免疫组织化学及Westernblot等方法检测结直肠癌组织及癌旁正常黏膜组织中的Desmin表达情况，分析Desmin表达与结直肠癌临床病理参数及预后的相关性。结果显示，Desmin在结直肠癌组织和癌旁正常黏膜组织中的表达水平存在明显差异，其在结直肠癌组织中表达水平明显升高。Chi—square分析结果显示，Desmin的表达与T分期（P〈0．05）、N分期（P〈0．05）、M分期（P〈O．05）、TNM分期（P〈0．05）、分化程度（P〈0．05）以及术前血CEA水平（P〈0．05）呈正相关，与性别、年龄、肿瘤位置以及术前血CA19—9水平无明显相关性（P〉0．05）。Knplan—Meier生存分析显示，与Desmin高表达患者相比，Desmin低表达患者总的3年生存率（3-yearOS）明显增高（Log—rank检验，P〈0．001）。结果表明，Desmin与结直肠癌的发生发展密切相关，并可能成为一种潜在性的肿瘤标志物。

This study was to explore the expression of Desmin in colorectal cancer tissue and its clinical significance;detected the expression of Desmin in colorectal cancer tissue and adjacent cancer normal mucosa tissue by the method of tissue microarray, immunohistochemistry, and Western blot; analyzed the relationship between the Desmin expression and clinicopathologieal parameters ＆ prognosis of colorectal cancer. Results show that there was obvious difference on the Desmin expression in colorectal cancer tissue and adjacent cancer normal mucosa tissue （ P 〈0.05） and the expression in colorectal cancer tissue was significantly higher. Chi-square analysis results show that there was positive correlation between Desmin expression and T stage （ P 〈0.05）, N stage （ P 〈0.05）, M stage （ P 〈0.05）,TNM stage （ P 〈0.05）,differentiation degree （ P 〈0.05） ＆ preoperative serum CEA level （ P 〈0.05）; but no significant correlation between Desmin expression and sex, age, tumor location ＆ preoperative serum CA19-9 （P 〉 0.05）. Kaplan-Meier survival analysis show that compared with Desmin high expression patients,the total 3-year survival rate （3-year OS） of Desmin low expression patients was significantly higher （ Log-rank detection, P〈0.001）.In conclusion,there is close relationship between Desmin and the occurrence ＆ development of colorectal cancer and Desmin probably become a potential tumor marker.