痰热清注射液对COPD大鼠体内炎症因子及气道黏液高分泌的影响

Effect of Tanreqing Injection on Inflammatory Factor and Airway Mucus Hypersecretion in COPD Rats

目的:观察痰热清注射液对慢性阻塞性肺疾病(COPD)大鼠白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)及黏蛋白5AC(MUC5AC)表达的影响,初步探讨痰热清注射液通过炎性介质路径干预COPD大鼠气道黏液高分泌的作用机制。方法:清洁级雄性SD大鼠60只,随机分为空白组、模型组、痰热清组,每组20只,除空白组外,其余2组采用脂多糖(LPS)联合烟熏建立COPD大鼠模型。痰热清组腹腔注射痰热清注射液,空白组和模型组同时给予等量生理盐水腹腔注射。检测大鼠肺功能,肺组织切片行HE染色,测定肺组织匀浆中IL-8、TNF-α及肺泡灌洗液中MUC5AC的含量。结果:与空白组比较,模型组和痰热清组大鼠体量明显下降,模型组气道阻力(RI)和肺总量(TLC)显著升高,第0.1秒用力呼气量与用力肺活量的比值(FEV0.1/FVC)显著降低,肺泡灌洗液中MUC5AC的含量和肺组织匀浆中的炎症因子IL-8、TNF-α显著升高,差异均有统计学意义(P<0.01);肺动态顺应性(Cdyn)无显著变化。与模型组比较,痰热清组大鼠体量增加,RI和TLC下降,FEV0.1/FVC升高,MUC5AC和TNF-α含量显著下降,差异均有统计学意义(P<0.01);IL-8和Cdyn无显著变化。结论:痰热清注射液可以通过抑制炎症介质的释放、调节MUC5AC分泌,来减轻气道炎症,从而改善气道黏液高分泌症状,在一定程度上起到延缓肺功能恶化的作用。

Objective：To observe the effect of Tanreqing injection on expressions of interleukin- 8（IL- 8）,tumor necrosis factor- α（TNF- α） and mucoprotein 5AC（MUC5AC） of rats with chronic obstructive pulmonary disease（COPD）,and to discuss preliminarily mechanism of Tanreqing injection intervening COPD rats airway mucus hypersecretion via inflammatory medium path. Methods： Selected 60 cases of class grade male SD rats, and divided them into the blank group, model group,Tanreqing group randomly, 20 cases in each group, and established COPD rats models with lipopolysaccharide（LSP）combined with smudging. Tanreqing group received Tanreqing injection from enterocoelia,at the same time blank group and model group received equivalent normal saline from enterocoelia. Detected lung function of rats,cut lung tissue into slice and HE stained, detected levels of IL- 8, TNF- α and MUC5 AC in bronchoalveolar lavage fluid of lung tissue homogenate.Results： Comparing with blank group, rats body mass of model group and Tanreqing group were decreased obviously,model group＇s airway resistance（RI） and total lung capacity（TCL） were increased obviously,ratio of forced expiratory volume at0.1 second and forced vital capacity（FEV 0.1/FVC） were decreased obviously,levels of MUC5 AC in bronchoalveolar lavage fluid and inflammatory factor IL- 8 and TNF- α in lung tissue homogenate were increased obviously in model group（P 〈0.01）.There was no obvious change on lung dynamic compliance（Cdyn）. Comparing with model group,rats body mass of Tanreqing group was increased,RI and TLC decreased,FEV0.1/FVC increased,levels of MUC5 AC and TNF- α decreased obviously,differences being significant（P 〈0.01）. There were no obvious changes on IL- 8 and Cdyn. Conclusions：Tanreqing injection can relieve airway inflammation by restraining release of inflammatory mediator and adjusting MUC5 AC secretion,then improve airway mucus hypersecretion symptom and delay lung function deterioration to some extent.