头针对脑缺血再灌注大鼠脑微血管层粘连蛋白影响的动态研究

Dynamic Study on Regulation of Scalp Acupuncture on Microvascular Laminin Expression in Rats with Cerebral Ischemia Reperfusion

目的:动态观察头针对脑缺血再灌注大鼠脑微血管层粘连蛋白的影响,以探讨头针抗脑缺血再灌注损伤的可能作用机制。方法:130只SD大鼠,随机分为假手术组、模型组和针刺组。采用改良的Zea Longa线栓法制作脑缺血再灌注模型,针刺组以头穴透刺的方法,选取百会(Du20)透曲鬓(GB7)针刺治疗30 min,每12 h针刺1次。应用免疫组化和免疫印迹检测方法,比较3组大鼠不同时间点脑微血管层粘连蛋白阳性表达与含量的变化。结果:模型组大鼠各时间点脑微血管层粘连蛋白表达较假手术组明显降低;针刺组在再灌注4、12 h,梗死灶周围区的层粘连蛋白阳性表达及含量均与模型组相似(P>0.05);再灌注24、48、72 h较模型组增加(P<0.05),但仍低于假手术组(P<0.05)。结论:早期头针治疗能增加缺血侧脑微血管层粘连蛋白的表达,并随着针刺治疗时间的延长,其效果明显提高。通过上调层粘连蛋白保护血脑屏障,可能是头针拮抗脑缺血再灌注损伤的作用途径之一。

Objective： To investigate the effect of scalp acupuncture on microvascular Laminin expression in the rats with cerebral ischemia reperfusion for exploring the interaction mechanisms. Methods： 130 Male SD rats were randomly divided into three groups： sham operation group,model group and scalp acupuncture group. Cere-bral ischemia / reperfusion model was induced by modified Longa＇s method. Scalp acupuncturing group was treated with Baihui- Qubin（ Du20- GB7） acupuncture for 30min,once per 12 h. The positive expression and protein content of Laminin were determined by immunohistochemistry and Western blot Methods. Results： Laminin expression and content in model group rats＇ brain at each time- point were lower to compare with sham group,the expression and content of Laminin about infarction in the scalp acupuncture group were similar with the model group in IR4 h and IR12 h（ P〈0. 05）,but which were higher obviously than those of model group in IR24 h,IR48 h and IR72 h（ P〈0. 05）,and in the same time-point,the expression and content of Laminin were still lower than those in the sham group（ P〈0. 05）. Conclusion： Scalp- acupuncture therapy can promote the expression of Laminin in rats with acute cerebral ischemia /reperfusion,and that was further increased with the treatment time prolonging. Scalp- acupuncture therapy can ameliorate cerebral ischemia reperfusion injury by possibly enhancing Laminin expression and protecting blood-brain barrier.