黄芩素联合亚高温热疗对人肺腺癌A549细胞抑制作用的体外研究

Study on Inhibition Effect of Baicalein Combined with Mild Hyperthermia on Proliferation of Lung Adenocarcinoma Cell Line A549 in Vitro

目的:观察黄芩素联合亚高温热疗对人肺腺癌A549细胞的体外抑制作用并探讨其可能的作用机制。方法:以体外培养的肺腺癌A549细胞为研究对象,CCK-8法检测黄芩素联合亚高温热疗对A549细胞增殖的抑制作用,流式细胞仪检测黄芩素联合亚高温热疗对A549细胞周期的影响,Western blot法检测黄芩素联合亚高温热疗对A549细胞HSP70(热休克蛋白70)蛋白表达的影响。结果:不同浓度的黄芩素均能有效地抑制A549的细胞增殖,黄芩素联合亚高温热疗具有更好的细胞抑制作用;联合亚高温热疗处理后,G2/M期细胞含量明显增加。黄芩素联合亚高温热疗组A549细胞的HSP70蛋白表达明显降低,与热疗组比较,差异显著(P<0.05)。结论:亚高温热疗能增强黄芩素对A549细胞的体外抑制作用。

Objective： To investigate the inhibition effect of Baicalein combined with mild hyperthermia（ MHT） on proliferation of lung adenocarcinoma cell line A549 and explore the possible mechanism. Methods：Lung adenocarcinoma cell line A549 cultured in vitro were taken as models,the inhibition effect of Baicalein combined with mild hyperthermia（ MHT） on proliferation of lung adenocarcinoma cell line A549 was detected by CCK- 8 method,changes of A549 cell cycle were determined by FCM.,the expression of HSP70 protein was detected by Western blot. Results： The growth of A549 cells was inhibited by Baicalein at different concentration,the inbihition effect of Baicalein combined with mild hyperthermia was more significant.. A549 cell cycle trended to be arrested in the G2 / M phase. The expressionof HSP70 protein decreased after cultured with Baicalein combined with mild hyperthermia to compare with MHT group. Conclusion： Baicalein combined with mild hyperthermia could inhibit the proliferation of lung adenocarcinoma cell line A549 in vitro.