慢性牙周炎患者龈沟液中IL-8和TNF-α水平变化及临床意义

Changes of IL-8 and TNF-α Levels in Gingival Sulcus Fluid of Patients with Chronic Periodontitis and Theirs Clinical Significance

目的:探讨慢性牙周炎患者龈沟液中白细胞介素-8(IL-8)和肿瘤坏死因子-α(TNF-α)水平变化及临床意义,为临床诊疗提供参考。方法:选择2013年12月到2015年12月我院收治的60例慢性牙周炎患者为实验组,选择同期60例牙周健康者为对照组。对照组对象于体检时、实验组患者于牙周常规治疗前后收集龈沟液并记录牙龈指数(GI)、牙龈沟出血指数(SBI)、菌斑指数(PLI)、牙周袋探诊深度(PD)、临床附着丧失(CAL)等牙周临床指标。测量并比较两组对象龈沟液中IL-8和TNF-α水平。结果:实验组患者治疗前GI、SBI、PLI、PD及CAL等牙周临床指标均明显高于对照组,差异具有统计学意义(P<0.05);治疗后,实验组患者各牙周临床指标明显低于治疗前,差异有统计学意义(P<0.05),但与对照组比较差异无统计学意义(P>0.05)。实验组患者治疗前龈沟液中IL-8和TNF-α水平均明显高于对照组,差异有统计学意义(P<0.05);治疗后,实验组患者龈沟液中IL-8和TNF-α水平均明显低于治疗前,差异有统计学意义(P<0.05)。实验组患者治疗前龈沟液中IL-8水平与牙周临床指标PD呈正相关性(r=0.495,P=0.027),TNF-α水平与牙周临床指标SBI、PD呈正相关性(r=0.512,0.673;P=0.019,0.012)。结论:慢性牙周炎患者龈沟液中IL-8和TNF-α具有较高水平,两者可能与慢性牙周炎的发生发展有关,对于临床诊断慢性牙周炎具有积极的临床意义。

Objective： To investigate the changes of interleukin-8 （IL-8）and tumor necrosis factor-a （TNF-a）levels in gingival sulcus fluid of patients with chronic periodontitis and theirs clinical significance, to provide reference for clinical diagnosis and treatment. Methods： Selected 60 cases of patients with chronic periodontitis who were collected and treated in our hospital from December 2013 to December 2015 as experiment group, and 60 cases of patients with periodontal health as control group at the same time. Collected the gingival sulcus fluid and recorded the gingival index（GI）, gingival sulcus bleeding index（SBI）, plaque index（PLI）, probing depth（PD） and clinical attachment loss （CAL） at the times of physical examination of the control group, before and after the routine periodontal therapy of patients in the experimental group. Measured and compared the IL-8 and TNF-a levels between the patients of two groups. Results： The GI, SBI, PLI, PD and CAL periodontal clinical indexes of patients in experimental group before treatment were significantly higher than the control group, the difference was statistically significant （P〈0.05）; After treatment, the periodontal clinical indexes of patients in experimental group were significantly lower than before treatment, the difference was statistically significant （P〈0.05）, but there was no significant difference compared with the control group （P〉0.05）. The IL-8 and TNF-e~ levels in gingival sulcus fluid of patients in experimental group before treatment were significantly higher than the control group, the differences were statistically significant（P〈0. 05）; After treatment, the IL-8 and TNF-a levels in gingival sulcus fluid of patients in experimental group were significantly lower than before treatment, the differences were statistically significant（P〈0.05）. The IL-8 levels in gingival sulcus fluid of patients in experimental group before treatment have positively correlation with PD of periodontal clinical indicators （r=0.495, P=0.027）, the TNF-a levels have positively correlation with SBI and PD of periodontal clinical indicators （r=0.512, 0.673; P=0.019, 0.012）. Conclusion： The IL-8 and TNF-a in gingival sulcus fluid of patients with chronic periodontitis have high levels, which may be associated with the development of chronic periodontitis, and it has positive clinical significance for clinical diagnosis of chronic periodontitis.