非小细胞肺癌患者人乳头瘤病毒感染分析

Analysis of human papillomavirus infection in non-small cell lung cancer

目的：研究非小细胞肺癌（NSCLC）患者人乳头瘤病毒（HPV）感染情况，探讨 NSCLC HPV感染与临床病理特征的相关性。方法收集156例病理确诊的 NSCLC 石蜡组织标本，采用 Luminex 液相芯片技术对所有标本进行 HPV 基因分型。收集患者临床病理资料，分析 HPV 感染与临床病理特征的相关性。结果156例 NSCLC 患者组织中 HPV 感染率为25．6％（40／156），其中高危型37例，包括HPV16、18、33、58亚型。HPV 感染与患者性别（χ^2＝4．387，P ＝0．036）、吸烟史（χ^2＝8．130，P ＝0．004）、组织类型（χ^2＝4．075，P ＝0．044）和淋巴结转移（χ^2＝7．082，P ＝0．008）相关；与患者年龄（χ^2＝0．013， P ＝0．910）、分化程度（χ^2＝1．727，P ＝0．189）、临床分期（χ^2＝0．179，P ＝0．672）、远处转移（χ^2＝3．012， P ＝0．083）无关。Logistic 回归模型分析发现患者淋巴结是否转移是 HPV 感染的独立影响因子（OR ＝0．384，95％CI 为0．153～0．967，P ＝0．042）；而患者性别（OR ＝1．402，95％CI 为0．522～3．769，P ＝0．503）、吸烟史（OR ＝0．506，95％CI 为0．194～1．322，P ＝0．506）、组织类型（OR ＝0．393，95％CI 为0．133～0．161，P ＝0．091）不是 HPV 感染的独立影响因子。结论我国部分 NSCLC 患者存在 HPV 感染，HPV 感染可能与肺癌的发生发展密切相关。

Objective To detect the infection of human papillomavirus （HPV）in patients with non-small cell lung cancer （NSCLC）and explore the relationship between HPV infection and clinicopathological features.Methods HPV detection and genotyping in 156 cases of NSCLC were performed using a new liquid chip based on Luminex.Patient clinical characteristics were also recorded,and the relationship between HPV infection and clinicopathological features was studied.Results Of the 156 tumor-DNA samples tested,40 （25.6%）cases showed presence of HPV-DNA,of which 37 cases were of a high-risk HPV type （16,18,33, 58）.The differences were statistically significant between the HPV-positive and HPV-negative groups in gender （χ^2 =4.387,P =0.036）,smoking （χ^2 =8.130,P =0.004）,histologic type （χ^2 =4.075,P =0.044）and lymph node metastasis （χ^2 =7.082,P =0.008）.The differences were not statistically significant between the HPV-positive and HPV-negative groups in age （χ^2 =0.013,P =0.910）,differentiated degree （χ^2 =1.727, P =0.189）,clinical stages （χ^2 =0.179,P =0.672）,distant metastasis （χ^2 =3.012,P =0.083）.Logistic regression analysis revealed that lymph node metastasis alone was an independent predictive factor of HPV infection in NSCLC （OR =0.384,95%CI：0.153-0.967,P =0.042）,and gender （OR =1.402,95%CI：0.522-3.769,P =0.503）,smoking （OR =0.506,95%CI：0.194-1.322,P =0.506）,histologic type （OR =0.393,95%CI：0.133-0.161,P =0.091）were not independent predictive factors of HPV infection. Conclusion The infection of HPV presents in part of Chinese NSCLC patients,and HPV infection may be connected with occurrence and development of lung cancer.