IMP3在放化疗前后宫颈鳞状细胞癌的表达及临床意义

Expressions of IMP3 in cervical squamous cell carcinoma before and after the therapy of radiation and chemotherapy

目的：探讨胰岛素样生长因子ⅡmRNA 结合蛋白3（IMP3）在不同级别宫颈上皮内病变（SIL）及放化疗前后宫颈鳞状细胞癌（CSCC）组织中的表达及临床病理意义。方法采用免疫组织化学法检测 IMP3蛋白在80例 CSCC 组织、90例 SIL（60例高级别、30例低级别）以及30例宫颈炎组织中的表达情况，分析 IMP3表达与 CSCC 临床病理特征的关系。结果 IMP3在 CSCC 组织、高级别 SIL、低级别 SIL、宫颈炎组织中的表达率分别为86．3％（69／80）、78．3％（47／60）、33．3％（10／30）、0（0／30），4组间差异有统计学意义（χ^2＝87．01，P ＜0．01）。放化疗后 IMP3的表达率较放化疗前降低，差异具有统计学意义（60．0％∶85．0％；χ^2＝5．79，P ＝0．013）。IMP3的表达与淋巴结转移（χ^2＝3．97，P ＝0．046）、分化程度（χ^2＝5．95，P ＝0．018）、临床分期（χ^2＝5．82，P ＝0．016）、浸润深度（χ^2＝5．73，P ＝0．017）相关，而与患病年龄无关（χ^2＝0．11，P ＝0．745）。结论 IMP3在 CSCC 组织中高表达，并与癌组织分化程度及浸润进展相关，放化疗可降低其表达。

Objective To discuss the expressions and clinicopathologic significances of insulin-like growth factor Ⅱ mRNA binding protein 3 （IMP3）in squamous intraepithelial lesion （SIL）and cervical squa-mous cell carcinoma （CSCC）before and after the therapy of radiation and chemotherapy.Methods The expressions of IMP3 in 80 cases of CSCC,90 cases of SIL （60 cases of HSIL,30 cases of LSIL）and 30 cases of cervicitis were detected by immunobistochemistry.The relations between IMP3 and clinicopathological cha-racteristics of CSCC were analyzed.Results The expression rates of IMP3 in CSCC,HSIL,LSIL and cervici-tis were 86.3%（69 /80）,78.3%（47 /60）,33.3%（10 /30）and 0（0 /30）,and the difference among the four groups was statistically signicant （χ^2 =87.01,P 〈0.01）.The positive expression rate of IMP3 declined by radiation or chemotherapy （60.0% vs.85.0%,χ^2 =5.79,P =0.013）.The expression of IMP3 was related with lymph node metastasis （χ^2 =3.97,P =0.046）,differentiated degree （χ^2 =5.95,P =0.018）,clinical stage （χ^2 =5.82,P =0.016）and invasion depth （χ^2 =5.73,P =0.017）.There was nothing to do with age （χ^2 =0.11,P =0.745）.Conclusion IMP3 expresses excessively in CSCC,and is associated with pathologi-cal grade and invasion progress.Radio-chemotherapy can reduce the expression of IMP3.