男性淋病奈瑟球菌3种不同检测方法的对比研究

Comparative study on three test methods for Neisseria gonorrhoeae

目的对比研究3种检测方法在淋病奈瑟球菌诊断中的临床应用价值。方法收集370例疑似淋病奈瑟菌感染男性患者的尿道分泌物和尿液标本,分别进行以下3种方法检测:普通培养法、DNA实时荧光定量法(RT-PCR)及RNA实时荧光恒温扩增检测(SAT)法。以普通培养法作为对照组,对比3组检测方法对淋病奈瑟菌的阳性率以及检测淋球菌的符合度情况。结果普通培养法、RT-PCR、SAT法阳性检出率分别为24.1%、11.8%、24.8%;普通培养法与SAT法淋病奈瑟菌阳性率均高于RT-PCR法,差异有统计学意义(P<0.05);普通培养法与SAT法淋病奈瑟球菌阳性率差异无统计学意义(P>0.05)。结论在临床工作中对男性淋病奈瑟球菌进行诊断时,建议选择2种以上的检测方法以提高阳性检出率;SAT法阳性反映活细菌数,可以更好地指导临床抗生素合理使用。

Objective To comparatively discuss the clinical application value of three test methods in the detection of Neisseria gonorrhoeae. Methods Urethral secretion and urine samples were collected from 370 cases of suspected male patients with Neisseria gonorrhoeae infection and given the following three detection methods respectively,including cultivating assay method,DNA real-time polymerase reaction method（ RT-PCR） and RNA real-time fluorescent constant temperature amplification detection（ SAT） method. Results The positive rates of common culture method,RT-PCR method and SAT were respectively24. 1%,11. 8%,24. 8%; the positive rate of RT-PCR was higher than that of the common culture method and SAT method in the detection of Neisseria gonorrhoeae,with the differences statistically significant（ P 〈 0. 05）; there was no statistical significance on the positive rate difference between the cultivation method and SAT method in the detection of Neisseria gonorrhoeae（ P 〉 0. 05）. Conclusion In the diagnosis of Neisseria gonorrhoeae in male in clinical work,more than two detection methods are recommended to improve the positive rate; SAT positive can reflect the living bacteria number,and can better guide the clinical rational use of antibiotics.