缺铁胁迫对小豆幼苗生理特性的影响

Effect of Iron Deficiency on Physiological Characteristics in Seedlings of Adzuki Beans(Vigna angularis)

为了探讨小豆铁高效的生理指标,研究缺铁胁迫对小豆(Vigna angularisi)幼苗主要生理特性的影响。选取4个小豆材料(13MY001、13MY007、FMY020及JN6)幼苗,采用溶液培养方法,设置Fe(Ⅱ)-EDTA浓度为0 mol/L、4×10-5 mol/L两组处理,分别于处理后7、14、21 d对其相关指标进行测定分析。结果表明,缺铁胁迫降低了小豆幼苗叶片的叶绿素含量及光合速率,增加了小豆幼苗根系呼吸速率及Fe3+还原酶活性,并且在+Fe和-Fe处理间差异达显著水平(P<0.05);不同小豆品种(品系)间的上述指标均存在显著差异。综合分析得出,小豆的幼苗叶片的叶绿素含量、光合速率及根系Fe3+还原酶活性、根系呼吸速率可作为筛选、培育铁高效小豆品种的生理指标。

To investigate the effects of iron deficiency on physiological characteristics in seedlings of Adzuki Beans（Vigna angularis）,four accessions of Adzuki Beans（13MY001,13MY007,FMY020 and JN6）were cultured in solution with or without Fe（Ⅱ）-EDTA.Some traits were analyzed in 7,14,and 21 days. The results show that the iron deficiency significantly reduces the chlorophyll content,photosynthetic rate,root surface area,total root length and total biomass,and increase the Fe（Ⅲ）reductase activity and root respiration rate of Adzuki Beans seedlings. Furthermore,the differences between different cultivars are significant. The results obtained in this experiment indicated that the combination of the chlorophyll content,photosynthetic rate,and Fe（Ⅲ）reductase activity and root respiration rate could be a promising screning index for selecting of Fe-efficient genotypes in Adzuki Beans.