摘要
目的通过检测肝泡型包虫病(hepatic alveolar echinococcosis,HAE)病灶边缘带肝组织中Ⅰ型和Ⅲ型胶原(typeⅠand typeⅢcollagen)蛋白的表达,探讨其与HAE进展中生物学行为的关系。方法选取2012年1月至2014年12月期间青海大学附属医院肝胆胰外科HAE患者手术切除的病灶边缘带肝组织及其距病灶5 cm以外的正常肝组织标本各20例,进行HE和Masson染色,光镜下观察HAE病灶周围肝脏组织的病理学改变和纤维化程度;采用免疫组化染色方法检测肝组织中Ⅰ型和Ⅲ型胶原蛋白的表达。结果病灶边缘带肝组织的纤维化程度为Ⅱ级,正常肝组织的纤维化程度为0级,其差异有统计学意义(P<0.05)。Ⅰ型及Ⅲ型胶原蛋白在病灶边缘带肝组织的显色指数分别为7.45±1.85和8.00±1.62,均高于正常肝组织的3.10±1.02和3.50±0.89,其差异均有统计学意义(t=-9.21,P=0.001;t=-10.88,P=0.001)。结论 HAE患者病灶周围组织存在肝纤维化,HAE可能通过促进Ⅰ型和Ⅲ型胶原蛋白的表达进而诱导肝纤维化的发生。
Objective To investigate the expressions of type Ⅰ and type Ⅲ collagen protein in hepatic alveolar echinococcosis tissues, and to explore its relationship with the biological behavior in progress of hepatic alveolar echinococcosis (HAE). Methods Twenty samples of normal liver tissues and liver tissues at the edge of the lesion with HAE in our hospital from Jan. 2012 to Dec. 2014 were collected, and HE and Masson staining were performed. The pathological changes and the degree of fibrosis of liver tissues around HAE lesion were observed under light microscope. The expressions of type Ⅰ and type Ⅲ collagen protein in liver tissues were detected by immunohistochemical staining. Results The degree of liver fibrosis of liver tissues at the edge of the lesion with HAE was grade Ⅱ, and the degree of fibrosis of normal liver tissues was grade 0, the difference between the two was statistically significant (P〈0.05). The color index of type I and type Ⅱ collagen protein in the liver tissues at the edge of the lesion with HAE was 7.45±1.85 and 8.00±1.62, respectively, which were higher than those of normal liver tissues (3.10±1.02 and 3.50±0.89), the difference were statistically significant (t=-9.21, P=0.001 ; t=-10.88, P=0.001 ). Condusions There is liver fibrosis around the lesion in the patients with HAE. HAE may promote the expressions of type Ⅰ and type Ⅲ collagen and then induce the occurrence of liver fibrosis.
作者
阳丹才让
宋戈
张灵强
游志远
任利
侯立朝
王海久
周瀛
温浩
樊海宁
YANGDAN Cairang SONG Ge ZHANG Ling-qiang YOU Zhi-yuan REN Li HOU Li-chao WANG Hai-jiu ZHOU Ying WEN Hao FAN Hai-ning(Department of Hepatopancreatobiliary Surgery, The Affiliated Hospital of Qinghai University, Xining 810001, Qinghai Province, China The Key Laboratory of Hydatid Disease Research in Qinghai Province, Xining 810001, Qinghai Province, China The First Affiliated Hospital of Xinjiang Medical University, Ururnqi 830054, Xinjiang Uygur Autonomous Region, China)
出处
《中国普外基础与临床杂志》
CAS
2016年第10期1209-1213,共5页
Chinese Journal of Bases and Clinics In General Surgery
基金
青海省科技厅国际合作项目(项目编号:2013-H-803)
青海省科技厅项目(项目编号:2012-N-536)
青海省临床重点建设专科--肝胆胰外科
青海大学昆仑学者项目
2015年青海省卫计委指导性课题~~
关键词
肝泡型包虫病
多房棘球绦虫
胶原蛋白
肝纤维化
Hepatic alveolar echinococcosis
Echinococcus multilocularis
Collogen protein
Hepatic fibrosis