摘要
目的探讨高分辨率熔解曲线法用于检测新疆维吾尔族白癜风患者肿瘤坏死因子alpha rs1800629基因多态性的可行性。方法应用高分辨率熔解曲线法检测100例新疆维吾尔族白癜风患者肿瘤坏死因子alpha基因启动子区rs1800629位点多态性,并与PCR产物直接测序法得到的结果进行比较分析。结果 HRM法检测rs1800629位点多态性的灵敏度可以达到5%,HRM分型结果与PCR产物直接测序法结果相同,二者符合率100%。结论 HRM法具有比较好的灵敏度与准确性,是一种快速、简便、高通量检测白癜风患者TNF-α基因多态性的优选方法。
Objective To explore the practicability of detecting the single nucleotide polymorphism （SNP） of tumor necrosis factor-alpha （TNF-α） rs1800629 in Uygur patients with vitiligo by high resolution melting （HRM） assay. Methods HRM assay was employed to detect the SNP of rs1800629 site in promoter region of TNF-α in 100 Uygur patients with vitiligo, and the detection results were analyzed and compared with the direct sequencing results of polymerase chain reaction （PCR） products. Results The sensitivity of rs1800629 SNP could reach 5% by HRM assay. The results of HRM assay were consistent with those of the direct sequencing of PCR products, with the coincidence rate of 100%. Conclusions HRM assay has a high sensitivity and accuracy, and it is a rapid, simple, high-throughput and optimal method for the detection of TNF-α SNP in patients with vitiligo.
出处
《实用预防医学》
CAS
2016年第11期1302-1305,共4页
Practical Preventive Medicine
基金
新疆维吾尔自治区自然科学基金(2015211C215)
