高效液相色谱法测定新疆地区甜叶菊中甜菊苷和莱鲍迪苷A的含量

Content determination of Stevioside and Rebaudioside-A in Stevia rebaudiana Bertoni in Xin Jiang province by HPLC

目的建立测定新疆地区甜叶菊中甜菊苷和莱鲍迪苷A含量的方法。方法采用高效液相色谱法,色谱柱为Agilent·Zorbax·SB-C_（18）（4.6 mm×250 mm,5μm）,流动相为乙腈-磷酸钠缓冲液（32∶68 v/v.p H=2.60）,流速为1.0 m L/min;检测波长为210 nm。结果甜菊苷和莱鲍迪苷A进样量分别在0.426～2.130μg、1.816～5.448μg范围内与峰面积积分分值呈良好线性关系（r=0.999 9）,二者精密度、稳定性、重复性试验的RSD≤1.50%,平均加样回收率分别为99.98%、100.06%,RSD分别为0.86%、1.16%,测得甜叶菊中甜菊苷的含量为5.74 mg/g,莱鲍迪苷A的含量为10.80 mg/g。结论高效液相色谱法简便、准确、重复性好,可用于新疆地区甜叶菊中甜菊苷和莱鲍迪苷A的含量测定。

Objective To establish the method for the content determination of Stevioside and Rebaudioside A in Stevia rebaudiana Bertoni in Xin Jiang province. Methods HPLC method was adopted. The determination was performed on Agilent·Zorbax·SB-C_（18）（ 4. 6 mm × 250 mm,5 μm） column with mobile phase consisted of acetonitrile-Sodium phosphate（ pH = 2. 60 32： 68 v / v） at the flow rate of 1. 0m L / min. The determination wavelength was set at 210 nm. Results The linear ranges of Stevioside and Rebaudioside-A were 0. 426 ~ 2. 130 μg,1. 816 ~ 5.448 μg（ r = 0. 999 9）,respectively. RSDs of precision,stability and reproducibility tests were all lower than 1.50%. Average recovery were 99. 98%（ RSD = 0. 86%）,100. 06%（ RSD = 1. 16%）,respectively. The average measured content of stevioside was 5. 74 mg / g. The average measured content of Rebaudioside A was 10. 8mg / g.Conclusion The method is easy,accurate and reproducible,and can be used for the determination of Stevioside and Rebaudioside-A in Stevia rebaudiana Bertoni in Xin Jiang province.