柱前衍生高效液相色谱法测定香烟中重金属离子的含量

Determination of Ni^(2+) and Cu^(2+) in Cigarette by Pre-column Deriving High Performance Liquid Chromatography

研究了柱前衍生高效液相色谱法测定香烟中Ni2+和Cu2+含量的方法.利用二乙基二硫代氨基甲酸钠(Na DDTC)进行柱前衍生,然后采用Hypersil ODS2 C-18反相色谱柱(5μm;250×4.6 mm)为固定相,甲醇∶水(体积比75∶25)为流动相进行检测.结果如下:Ni2+和Cu2+的线性范围为0.1~30μg·m L-1,线性相关系数为0.9988和0.9997,检出限为0.017μg·m L-1和0.011μg·m L-1,加标回收率为96.83%~104.50%.结果表明该方法操作简单快速,测定准确,检出限低,可作为其他检测金属离子方法的替代方法.

Ni（2＋）and Cu^（2 ＋）in cigarette were determined by the pre-column deriving HPLC method. The samples were derived by sodium diethyldithiocarbamate（ Na DDTC） before it was analyzed. The experiment used a Hypersil ODS2 C- 18 chromatography column（ 5μm; 250 × 4. 6mm） as stationary phase and Me OH： H2O（ 75：25,v / v） as mobile phase. The calibration curves for two heavy metal ions were linear in the range of 0. 1 ~ 30μg·mL- 1with linear correlation coefficients of 0. 9989 ~ 0. 9997. The limits of detection were 0. 017 μg·mL- 1and 0. 011μg·mL- 1,respectively,along with the recoveries of 96. 83% ~ 104. 50%. The established method including simple operation is accurate,rapid and stable to detect the two heavy metal ions in cigarette with low detection limit. The method can be used as an alternative method of detecting other metal ions.