丙泊酚对电离辐射引起小鼠造血系统损伤的防护作用

Protective effect of propofol on total body irradiation induced hematopoietic system injury in mice

目的观察丙泊酚对电离辐射引起造血系统损伤防护作用。方法实验采用3种照射剂量:生存率实验照射剂量为7.5 Gy、脾结节形成实验照射剂量为6 Gy、外周血细胞计数和骨髓单侧股骨细胞计数实验照射剂量为2 Gy;生存率实验分为4组:7.5Gy组、7.5Gy+5 mg/kg丙泊酚组、7.5Gy+10 mg/kg丙泊酚组、7.5Gy+20 mg/kg丙泊酚组。其余实验分为4组:对照组、丙泊酚20 mg/kg组、照射组(2Gy或6Gy)、照射+丙泊酚20 mg/kg组。给药方式为照射前1 d、照射当天提前30 min各给药一次和照射后7 d连续腹腔注射,每日1次,照射后第9天活杀小鼠,检测脾结节形成数,各类外周血细胞数及单侧股骨骨髓细胞数。结果丙泊酚20 mg/kg能提高受照射小鼠30 d生存率,增加受照射小鼠脾结节形成数、增加受照射小鼠外周血白细胞数、红细胞数、血红蛋白含量及小鼠单侧股骨骨髓有核细胞数,与照射对照组比较,结果有统计学意义(P<0.05)。结论丙泊酚对电离辐射引起的造血系统损伤均有保护作用,其作用机制有待进一步研究。

Objective To observe the protective effect of propofol on hematopoietic system injury in mice with total body irra-diation（TBI）. Method Three different radiation doses were used in the experiments：7.5 Gy TBI in 30 day-survival experiment,6Gy TBI in colony-forming unit spleen（CFU-S）experiment and 2Gy TBI in the other experiment;mice were divided into 4 groups in a 30 day-survival experiment,including 7.5Gy TBI group,7.5 Gy TBI＋5 mg/kg propofol group,7.5 Gy TBI＋10 mg/kg propofol group and 7.5 Gy TBI ＋ 20 mg/kg propofol group. For the other experiments,mice were divided into 4 groups：control group,propofol group,TBI（2 or 6 Gy）group,and TBI＋20 mg/kg propofol group. Propofol of 20 mg/kg were administered to mice 1 d before TBI,30 mins before TBI and once each day within the following 7 days after TBI. Mice were euthanized on the ninth day after TBI,the number of CFU-S,peripheral blood parameters and bone marrow cells per femur were measured in this experiment. Results Propofol im-proved the 30 day-survival of lethally irradiated mice. There were increases in number of CFU-S,white blood cells,red blood cells, hemoglobin in peripheral blood and bone marrow cells per femur in 2 Gy TBI＋20 mg/kg propofol group compared to 2 Gy TBI group （P〈0.05）. Conclusion Propofol exhibits a promising protective effect on TBI-induced hematopoietic system injury;further study should be focused on the related mechanisms.