摘要
以杂交节水抗旱稻‘旱优73'和‘旱优3号'及其亲本为材料,采取一种高通量96深孔板提取水稻DNA的方法进行杂交种子纯度鉴定。结果表明:该提取方法获得的DNA纯度高,能满足正常的SSR分析,利用引物RM208和RM336扩增的条带清晰可辨,可对‘旱优73'和‘旱优3号'杂交种子纯度进行准确鉴定。
The purity of rice hybrid seed was identificated by a method of high throughput DNA extraction in rice with deep-well plate,taking the hybrid water-saving and drought resistance rice ＇ Hanyou 73 ＇ and＇ Hanyou 3＇ and their parents as materials.The results showed that the purity of DNA obtained by the method was high and could meet the normal SSR analysis.The amplified bands by primer RM208 and RM336 were clearly,and the purity of hybrid seeds of ＇ Hanyou 73 ＇ and ＇ Hanyou 3 ＇ could be accurately identificated by the method.
作者
刘毅
赵洪阳
唐金娟
王加红
刘国兰
LIU Yi ZHAO Hong-yang TANG Jin-juan WANG Jia-hong LIU Guo-lan(Shanghai Agrobiological Gene Center, Shanghai 201106, China Shanghai Tiangu Biotechnology Co. , Ltd. , Shanghai 201203, China)
出处
《上海农业学报》
CSCD
2016年第5期8-10,共3页
Acta Agriculturae Shanghai
基金
国家高技术研究发展计划(863计划)项目(2012AA101102)
(2014AA10A604)
关键词
高通量
DNA提取
纯度鉴定
High throughput
DNA extraction
Purity identification
