室内外培养对耐温微藻Desmodesmus sp.F51细胞生长和叶黄素积累的影响

Effects of Indoor and Outdoor Cultivation Conditions on the Cell Growth and Lutein Accumulation of Thermo-tolerant Desmodesmus sp.F51

在室内培养耐温微藻F51,考察了培养温度、自来水及非灭菌培养基对细胞生长及叶黄素积累的影响,并与50 L柱状光生物反应器户外分批培养情况进行对比.结果表明,藻株在35℃下的生物质产率和叶黄素产率最大,分别为767.5和3.05mg/（L·d）.低温培养有利于叶黄素积累,但生长速率减缓.用自来水及非灭菌培养基培养时,藻株无明显的生长抑制及染菌现象,在13.0-27.6℃户外培养温度下,藻株最高生物质产率、二氧化碳固定率和叶黄素产率分别为236.3,444.2和1.17 mg/（L·d）.

The effects of temperature, local tap-water and culture medium without sterilization on cell growth and lutein production of Desmodesmus sp. F51 were investigated under indoor conditions. Outdoor batch cultivation of Desmodesmus sp. F51 was also investigated in 50 L cylindrical photobioreactor. The results showed that the best biomass productivity [767.5 mg/（L·d）] and lntein productivity [3.05 mg/（L·d）] occurred at a temperature of 35 ℃. A low temperature enhanced the lutein content but reduced cell growth rate. There is no significant growth inhibition and microbiological contamination were observed for Desmodesmus sp. F51 using local tap-water and culture medium without sterilization. Under the outdoor temperature of 13.0-27.6 ℃, the highest biomass productivity, CO2 fixation rate and lntein productivity of 236.3, 444.2 and 1.17 mg/（L·d） were obtained, respectively.