蒙药阿给炭对胃溃疡模型大鼠黏膜下组织重建影响的实验研究

Experimental study of Mongolian medicine charred Agei effect on the gastric ulcer model rat mucosal tissues reconstruction

目的研究蒙药阿给炭对应激型胃溃疡模型大鼠溃疡组织炎性细胞浸润及新生血管数、微血管密度生成的影响,探讨阿给炭对胃溃疡黏膜下组织重建的作用机制。方法采用水浸束缚应激方法复制急性胃溃疡大鼠模型,实验分为8组,即空白对照组、模型对照组、云南白药组(阳性药)、雷尼替丁组(阳性药)、阿给生药组、阿给炭药低、中、高剂量组。连续给药1周后,常规处死,解剖剪取胃部溃疡组织,HE染色观察炎性细胞浸润,CD34及Ⅷ因子法标记观察溃疡组织微血管密度及新生血管数。结果与正常对照组相比,模型组大鼠溃疡部位新生血管数、微血管密度有显著性差异(P<0.05),与模型组比较,阿给炭高剂量组新生血管数、阿给炭药低剂量组微血管密度有显著性差异(P<0.05)。结论胃溃疡模型大鼠溃疡组织存在新生血管数及微血管密度生成不足,而给予阿给炭治疗后能显著提高溃疡组织新生血管数生成及微血管密度。

Objective To study the Mongolian medicine charred Agei effect on the chronic ulcer tissues in the stress gastric ulcer model rats,inflammatory cell infiltration,number of new blood vessels and MVD,to investigate the molecular mechanism for charred Agei to promote the healing of gastric ulcers. Methods Rat models of stress ulcer were established by using water immersion-restraint stress（ WRS）,and rats were randomly divided into 8 groups,named control group,model group,Yunnanbaiyao group（ Positive drug）,Ranitidine group（ Positive drug）,Agei crude drug group,low dose charred Agei group,middle dose charred Agei group and high dose charred Agei group. Intragastric administration were given a week continuously,clipped stomach ulcer tissue of the conventional sacrificed animals,using HE staining for observation of inflammatory cell infiltration,using CD34 and Ⅷ factor labeling for observation of ulcer tissue microvessel density and the number of new vessels. Results Compared with the control group,model group rat ulcer site neovascularization number,micro vascular density have significant difference（ P 0. 05）,and compared with the model group,the neovascularization number of high dose charred Agei group,the micro vascular density of low dose charred Agei group have significant difference（ P 0. 05）. Conclusion The gastric ulcer model rat ulcer tissues exist number angiogenesis and microvessel density generate deficiency,given charred Agei to treatment can significantly improve the number of vascular ulcers and microvessel density generation