百脉根细胞亲环素电子克隆和生物信息学分析

In silico cloning and bioinformatics analysis of cyclophilin from Lotus corniculatus

目的通过电子克隆技术对百脉根细胞亲环素(cyclophilin)基因进行预测。方法以大豆cyclophilin序列为探针,基于NCBI中百脉根的EST数据库和CAP3在线软件进行序列拼接,利用生物信息学数据库及相关软件对其结构和功能进行预测分析。结果百脉根cyclophilin基因全长1 346 bp,包含771 bp的开放阅读框,编码256个氨基酸,该蛋白为亲水性蛋白。结论为进一步解释基因的分子功能奠定理论及实验基础。

Objective Using electronic cloning technology to predict cyclophilin gene of Lotus corniculatus. Methods Using glycine max cyclophilin sequence as probe sequence, based on EST sequence fi＇om NCBI and assembled by CAP3 sequence assembly programme, using bioinforrnatic database and related software, the structure prediction and fimction analysis were performed. Results The full length of cyclophilin gene was 1 346 bp, it contained a 771 bp ORF, encoding 256 amino acids, and the protein was a hydrophilic protein. Conclusion The study is intended to further explain the molecular genetic function theory and experimental basis.