臭灵丹紫外指纹图谱共有峰率和变异峰率双指标序列分析法

Common and Variant Peak Ratio Dual-index Sequence Analysis in UV Fingerprint Spectra of Laggera pterodonta

目的:利用共有峰率和变异峰率双指标序列分析法可以对2个以上中药材样品进行定性评价,阐明不同产区间的相似程度,为中药材真伪鉴定和品质评价奠定基础。方法:利用共有峰率和变异峰率2个指标,采用平均值、平滑和二次微分对光谱数据进行处理,考察臭灵丹三氯甲烷、无水乙醇、水3种极性溶剂提取物各波段的稳定性及重复性,计算臭灵丹不同提取物的共有峰率和变异峰率,对不同产区的臭灵丹进行定性评价。结果:臭灵丹在三氯甲烷、无水乙醇和水3种极性溶剂下分别提取30,50,40 min可达到最大提取率,在20 h内稳定性良好。文中按照共有峰率的大小,建立了不同的共有峰率和变异峰率双指标序列方法。指纹图谱显示,臭灵丹不同产区样品间最大共有峰率为78.57%,最小共有峰率为11.11%。

Objective： The common and variant peak ratio dual-index sequential analysis method could be used exactly in qualitative evaluation of more than two herb samples,to clarify the similarity level among the samples from different areas,and also provide foundation for authenticity identification and quality evaluation of herbs. Method： With the dual indexes of common peak ratio and variant peak ratio,mean value,smoothness and second differential methods were used for the treatment of UV spectrum data to investigate the stability and repeatability of various wave bands of three different polarity solvent extracts（ methenyl trichloride,absolute ethanol and water） of Laggera pterodonta. The common peak ratio and variant peak ratio for the different extracts of L.pterodonta were calculated,and qualitative evaluation was performed for L. pterodonta from different areas. Result：The results showed that methenyl trichloride,absolute ethanol and water had the highest extraction ratios at the extraction time of 30,50 and 40 min respectively,with good stability in 20 h. The different dual-index sequential methods of common peak ratio and variant peak ratio were established in the present study according to the size of common peak ratios. The fingerprint spectral data implicated that the highest common peak ratio was 78. 57%,and the lowest common peak ratio was 11. 11% among different areas of L. pterodonta.