秦苓液对尿酸性肾病大鼠肾功能及肾组织病理改变的影响

Effect of Qinling Liquid on Renal Function and Pathological Changes of Renal Tissue in Rats with Uric Acid Nephropathy

目的:观察秦苓液对尿酸性肾损害大鼠肾功能及肾组织病理改变的影响,探讨其对尿酸性肾病(UN)肾脏损伤的保护作用及作用机制。方法:采用腺嘌呤灌胃伴酵母饲料喂养的方法造模,将成模大鼠随机分为正常组,模型组,别嘌呤醇组(23.33 mg·kg^(-1)·d^(-1)),秦苓液大、中、小剂量组(36.4,18.2,9.1 g·kg^(-1)·d^(-1)),连续干预8周。分别于0,2,4,6,8周检测各组大鼠24 h尿蛋白(u PRO/24 h),血尿酸(s UA),血肌酐(s CR)和血尿素氮(BUN);干预6周和8周后分别处死各组大鼠1/2量,取肾脏进行苏木素-伊红和过碘酸雪夫氏染色,光镜下观察。结果:与正常组比较,模型组大鼠u PRO/24 h,s UA,s CR,BUN明显升高,肾组织出现较明显的病理改变;与模型组比较,秦苓液各剂量组大鼠u PRO/24 h,s UA,s CR,BUN有不同程度的降低,肾组织病理损伤明显减轻。结论:秦苓液能显著改善UN模型大鼠肾功能,减轻肾组织病理损伤,保护肾脏。

Objective： To observe the effects of Qinling liquid on renal function and pathological changes of renal tissue in rats with uric acid nephropathy,and to discuss its protection mechanism for renal injury.Method： The rat model was induced by gavaging adenine and feeding yeast. Model rats were randomly divided into the normal group,the model group,the positive group（ 23. 33 mg·kg^- 1·d^-1of allopurinol） and high-dose,medium-dose,low-dose groups of Qinling liquid（ 36. 4,18. 2,9. 1 g·kg^- 1·d^-1）,the normal group and the model group were daily gavaged with distilled water. At the timepoint of 0,2,4,6,8 weeks,the levels of serum uric acid（ s UA）,serum creatinine（ s CR）,blood urea nitrogen（ BUN） and 24 h urine protein（ u PRO/24h） were detected. At the end of 6thand 8thweek,half of rats in each group were sacrificed and the kidney was harvested for hematoxylin-eosin and periodic acid-schiff staining. Result： Compared with the normal group,the levels of u PRO/24 h,s UA,s CR and BUN in the model group were dramatically inceased,pathological changes of renal tissue were serious. Compared with the model group,the levels of u PRO/24 h,s UA,s CR and BUN in each group of Qinling liquid were deceased at different degrees,and the pathological changes of renal tissue were alleviated. Conclusion： In model rats of uric acid nephropathy,Qinling liquid can significantly improve renal function and relieve renal pathological damage for kidney protection.