量子点@SiO2微球的水相合成及其在流式分析技术中的应用

Quantum Dots@SiO_2 Microsphere Aqueous Synthesis and Application in Flow Cytometric Analysis

通过表面配体交换将巯基硅烷包覆在量子点(QDs)表面,并与过量的硅烷共水解制备出包覆QDs的荧光硅球。该方法通过化学键合避免了QDs的泄露以及QDs荧光性质的改变,而且微球表面富集大量的巯基,为后续的反应提供活性位点。研究了反应时间、温度和搅拌速度对荧光微球形成的影响,优化条件得到了尺寸均一、单分散性的荧光微球。将荧光微球用于流式细胞分析检测,微球的数量和荧光强度能够满足流式分析检测的需要,适合制备成编码微球。

By ligands exchange between 3-mercaptopropyltrimethoxysilane（MPTMS）and 3-mercaptopropionic acid（MPA）,quantum dots were encapsulated into the SiO2 microsphere to avoid the leakage.There are plenty of groups on the surface of microsphere that provide active sites for further modification.The fluorescent properties of quantum dots did not obviously changed encapsulating into microsphere.Through optimization of preparation conditions,such as reaction time,temperature and stirring rate,the monodispersed microspheres were synthesized with narrow size distribution.Furthermore,the quantum dots with orange fluorescence was applied into flow cytometric analysis,and showing possibility of coding.