东北岩高兰醋酸乙酯部位化学成分及其对大鼠酒精性脂肪肝作用研究

Chemical constituents of ethyl acetate extract of Empetrum nigrum var. japonicum and their effect on alcoholic fatty liver of rats

目的对东北岩高兰Empetrum nigrum var.japonicum地上部分70%乙醇提取物的醋酸乙酯萃取部位进行化学成分和对大鼠酒精性脂肪肝（AFL）作用的研究,为东北岩高兰的开发和利用提供科学依据。方法利用色谱分离技术和波谱解析技术进行分离纯化、结构分析及鉴定。采用45%的酒精ig方法对SD大鼠进行AFL造模。观察东北岩高兰醋酸乙酯部位对AFL大鼠肝指数、血清学、肝组织匀浆等指标的影响。结果从东北岩高兰地上部分70%乙醇提取物的醋酸乙酯萃取部位分离得到8个化合物,分别鉴定为β-谷甾醇（1）、熊果酸（2）、乌苏醇（3）、2′,4′-二羟基二氢查耳酮（4）、2′,4′-二羟基查耳酮（5）、7-羟基二氢黄酮（6）、4′-甲氧基-2′-羟基二氢查耳酮（7）、4,2′,4′-三羟基查耳酮（8）。与模型组比较,给予东北岩高兰醋酸乙酯部位提取物后,大鼠肝指数降低明显,血清中三酰甘油（TG）、总胆固醇（TC）降低,血清酶学丙氨酸转氨酶（ALT）、天冬氨酸转氨酶（AST）降低,肝组织匀浆中超氧化物歧化酶（SOD）、谷胱甘肽（GSH）升高。结论化合物1~4、6~8为首次从东北岩高兰中分离得到。东北岩高兰的醋酸乙酯部位对大鼠AFL具有治疗效果。

Objective To study the chemical constituents from the ethyl acetate extract fraction of 70% ethanol extract in the aerial parts of northeastern crowberry（Empetrum nigrum var. japonicum） and their effect on the alcohol fatty liver（AFL） of rats, and to provide the scientific basis for the use and development of the northeastern crowberry. Methods Chromatography separation technology and spectral analysis technology were used for the separation, purification, structure analysis, and identification. AFL model of SD rats was induced by 45% ethanol. To observe the effect of ethyl acetate extract in northeastern crowberry on the liver index, serology, and liver tissue homogenates in AFL of rats. Results Eight compounds were isolated and identified as β-sitosterol（1）, ursolic acid（2）, uvaol（3）, 2′,4′-dihydroxy dihydrochalcone（4）, 2′,4′-dihydroxy chalcone（5）, 7-hydroxy flavone（6）, 4′-methoxy-2′-hydroxyl-dihydroxy chalcone（7）, and isoliquiritigenin（8）. Compared with the model group, giving the ethyl acetate extract of northeastern crowberry could decrease the liver index of rats, reduce serum lipid metabolism of TG, TC, reduce the serum enzyme ALT, AST, liver homogenate in oxidative stress, and increase the activities of SOD and GSH. Conclusion Compounds 1—4, and 6—8 are isolated from E. nigrum var. japonicum for the first time. The effect of ethyl acetate extract in E. nigrum var. japonicum on AFL of rats is obvious.