丹参素和原儿茶醛对丹酚酸B降解规律的影响研究

Study on effect of danshensu and protocatechuic aldehyde on degradation of salvianolic acid B

目的建立定量测定多种丹参酚酸的高效液相色谱(HPLC)分析方法,考察丹参素和原儿茶醛对丹酚酸B降解规律的影响。方法应用HPLC技术对丹酚酸B降解样品中的4种酚酸进行定量分析,应用LC-MS技术剖析样品的化学物质基础,对加入丹参素和原儿茶醛前后的丹酚酸B样品进行研究。结果丹酚酸B降解产物主要有9种,包括丹参素、原儿茶醛、丹酚酸B的多种异构体、紫草酸以及多种紫草酸异构体。加入相同质量浓度的丹参素和原儿茶醛,原儿茶醛对丹酚酸B降解的保护作用更明显,紫草酸异构体I和II的峰面积随着初始原儿茶醛质量浓度的增大而有所增加。结论原儿茶醛对丹酚酸B降解的保护作用可能是依赖于其弱酸性,紫草酸异构体I和II的峰面积受原儿茶醛初始质量浓度的影响可能与反应平衡改变有关。

Objective To develop a method for the quantitative determination of several salvianolic acids by HPLC and investigate the influence of danshensu and protocatechuic aldehyde on the degradation process of salvianolic acid B（Sa B）. Methods HPLC method was applied to quantitative analysis on four components in the degradation sample of Sa B. HPLC-MS method was applied to tentatively characterizing the structure of the degradation products. The effect of danshensu and protocatechuic aldehyde on the degradation of Sa B were found out by the analysis of chromatogram and mass spectra of degradation samples. Results Nine degradation products were found in the degradation samples of Sa B, including danshensu, protocatechuic aldehyde, isomers of Sa B, lithospermic acid, and its isomers. Protocatechuic aldehyde had stronger protective effect on Sa B compared to danshensu at the same mass concentration. Additionally, the peak area of lithospermic acid isomers I and II increased when the initial concentration of protocatechuic aldehyde was increased. Conclusion The mild acidity of protocatechuic aldehyde might account for its stronger protective effect on Sa B, and the connection between protocatechuic aldehyde and lithospermic acid isomers I and II might relate to the change of reaction balance.