银莱汤对食积复合FM1流感病毒感染小鼠血清免疫球蛋白及细胞因子含量的影响

Influences of Yinlai decoction on serum immunoglobulin and cytokines of mice with dyspepsia combined with FM1 influenza virus infection

目的探讨银莱汤对食积复合流感病毒感染小鼠免疫功能的影响,为银莱汤药效机理的研究提供实验依据。方法雄性昆明小鼠90只,随机分为正常对照组、感染模型组、食积感染模型组、银莱汤高中低剂量组、双黄连组、小儿化食丸组、利巴韦林组共九组,每组10只。食积感染模型组及各治疗组均采用高蛋白、高热量饲料结合52%牛奶溶液灌胃的方法制作食积动物模型,感染组小鼠则采用流感病毒亚甲型鼠肺适应株流感病毒滴鼻处理,并予相应的治疗药物或生理盐水灌胃。实验结束后,取眼球血,采用ELISA法检测血清中免疫球蛋白及细胞因子的含量。结果与正常组比较,感染组、食积感染组各免疫球蛋白A、G、M及白细胞介素(interleukin,IL)-2、IL-10、肿瘤坏死因子(tumor necrosis factor-a,TNF-α)及干扰素-γ(interferon-gamma,IFN-γ)水平显著增高(P<0.05);而食积感染组的IL-4降低。与食积感染组比较,各治疗组免疫球蛋白A、G、M的水平降低,其中银莱汤低剂量组和利巴韦林组明显降低(P<0.05);银莱汤各治疗组细胞因子IFN-γ升高(P<0.05)。与正常组相比,银莱汤各治疗组免疫球蛋白A、G、M升高,但无统计学差异,而细胞因子IL-2、IL-10、TNF-α、IFN-γ水平显著升高(P<0.05)。银莱汤各剂量组与小儿化食丸组、双黄连组相比,免疫球蛋白A、G、M无统计学差异,银莱汤各剂量组与小儿化食丸组、双黄连组、利巴韦林组的细胞因子IL-4、IL-10、TNF-α相比无统计学差异。结论银莱汤可通过调节免疫球蛋白及细胞因子的分泌,有效调节小鼠免疫功能,进而发挥其抗病毒疗效。

Objective To explore the influenees of Yinlai decoction on immune function in mice with dyspepsia combined with FM1 influenza virus infection, and to compare the efficacy of Yinlai decoction with anti-viral Western medicine, and identify the efficacy of Yinlai decoction and its potential mechanism. Methods 90 male mice were randomly divided into normal group, infection group, intection and dyspepsia group, high, middle and low dose Yinlai decoction groups, Shuanghuanglian group, Xiaoerhuashiwan group, ribavirin group. A diet-induced mice model with high fat and high protein diet were built in infection and dyspepsia group and all the treating groups to build models of dyspepsia, and the mice were then infected FM1 influenza virus by nasal inhalation in those groups. The blood was taken by removalling eyeball, to detect the level of Immunoglobulin and Cytokines by ELISA. Results Compared with the normal group, the levels of IdA, IgG, IgM and IL-2, IL-10, TNF-α, INF-γ were increased significantly in infection group as well as infection and dyspepsia compound group（ P〈0.05 ） , but the level of IL-4 were decreased in infection and dyspepsia compound group. Compared with infection and dyspepsia compound group, the levels of IdA, IgG, IgM were decreased in all treating groups, and the low-dose of Yinlai decoction group and ribavirin group were decreased significantly（ P〈O. 05 ） , the level of INF-r was increased in each dose of Yinlai decoction group. Compared with the normal group, the levels of IdA, IgG, IgM were increased and the levels of IL-2, IL-10, TNF-α, INF-γ/were increased significantly （P〈0.05） in each dose of Yinlai decoction group. Comparing with Yinlai decoction group, the levels of IdA, IgG, IgM between Shuanghuanglian group and XiaoerhuashiWan group had no significant difference. And the level of IL-4, IL-10, TNF-α of treating groups had no significant difference. Conclusion Yinlai decoction can adjust immune functions in mice by balancing the secretion of immunoglobulins and cytokines, and then achieve antiviral efficacy.