不同产地栽培掌叶大黄中游离蒽醌、结合蒽醌和总蒽醌量的差异分析

Difference Analysis of Free Anthraquinones, Combined Anthraquinones and Total Anthraquinones in Cultivated Rheum palmatum L. in Different Producing Areas

目的:测定不同产地的栽培掌叶大黄药材中游离蒽醌、总蒽醌及结合蒽醌的含量,为其药材质量评价和资源合理利用提供科学依据。方法:采用Agela Venusil XBP-C18柱(4.6 mm×250 mm,5μm),以甲醇-0.1%磷酸溶液(75:25)为流动相,流速为1.0 m L/min,检测波长为254 nm,柱温为35℃,对11批掌叶大黄药材进行测定,并对测定结果进行聚类分析。结果:不同产地的栽培掌叶大黄均含有5种蒽醌类衍生物,甘肃礼县上坪质量最优。采用聚类分析能将不同产地的11批掌叶大黄药材分为3类。结论:本实验考察了不同产地掌叶大黄的质量差异,可为大理苍山地区掌叶大黄的质量评价及规范化种植提供参考。

Objective： To determine content of free anthraquinones, combined anthraquinones and total anthraquinones in cultivated Rheum palmatum L. in different producing areas, and to provide scientific basis for quality assessment and reasonable using. ethods：Agela Venusil XBP-C18 column（4.6 mm×250 mm, 5 μm）was used with the mobile phase of methanol-0.1% posphoric acid solution（75：25）at the flow rate of 1.0 mL/min, the detection wavelength was set at 254 nm and the column temperature was 35 ℃. To determine the content of cultivated Rheum palmatum L. from 11 different producing areas, cluster analysis was carried out for the results. Results： 5 kinds of anthraquinons exist in cultivated Rheum palmatum L. from different producing areas, in which cultivated Rheum palmatum L. from Lixian, Gangsu was the best. Cluster analysis divided cultivated Rheum palmatum L. from 11 different producing areas into 3 categories. Conclusion： This paper analyzes the quality of cultivated Rheum palmatum L. from different producing areas, it could provide reference for quality assessment and standardized planting of cultivated Rheum palmatum L. in Dali.