肝癌经TAE术后肝细胞中PPAR-α的表达

PPAR-α Expression of Hepatocytes after TAE in Liver Cancer

目的：探讨PPAR-α在肝癌经肝动脉栓塞术（TAE）后肝功能异常中的作用机制。方法：建立新西兰大白兔VX2肝癌动物模型26只，模型成功率为86%（24/28），将兔VX2肝癌动物模型随机分为栓塞组12只，造影组12只，TAE术后8 h各组兔同时处死，取肿瘤旁取正常肝组织，制作石蜡切片，进行HE染色以及免疫组化，检测PPAR-α在肝组织中的表达。结果：栓塞组阳性细胞数介于51%-85%，阳性评估为＋＋/＋＋＋，造影组阳性细胞数介于10%-30%，阳性评估为＋/＋＋。统计学分析表明TAE术后，癌旁肝组织中PPAR-α的表达明显高于造影组（P＜0.05）。结论：TAE后，PPAR-a在肿瘤周围肝细胞中的表达明显增加，可能机制为癌旁正常肝组织缺血缺氧，从而导致PPAR-α高表达。

Objective： To investigate the function mechanism of PPAR -α in liver cancer on abnormal liver function after transcatheter arterial embolization（TAE）. Methods： VX2 hepatic cancer model in 26 New Zealand white rabbits model were established with 86% successful rate（24/28）. VX2 hepatic cancer models in rabbits were randomly divided to embolization group（12）, angiography group（12）. Rabbits of very group were sacrificed 8 hours after TAE. Liver tissues surrounded HCC were collected to make paraffin section, and the expression of PPAR-α in hepatic tissue was detected after hematoxylin ＆ eosin（HE）staining and immunohistochemistry. Results： Positive cells in embolization group ranged from 51%-85% with a positive assessment ＋＋ / ＋＋＋;positive cells in angiography group were between 10%-30% with a positive assessment of ＋ / ＋＋. Statistical analysis showed that the PPAR-α expression in cancer adjacent hepatic tissue of embolization group was significantly higher than angiography group（P〈0.05）. Conclusion： After TAE, PPAR-α expression in tumor adjacent hepatic tissue increased significantly. The possible reason of high expression of PPAR-α might be hypoxia ischemia of cancer adjacent normal tissues.