根肿菌生理小种鉴定与甘蓝型油菜品种资源的抗性评价

Pathotype identification of Plasmodiophora brassicae and resistance of rapeseed variety resources to clubroot disease

为了鉴定评价油菜根肿病菌致病性的分化与甘蓝型油菜品种资源的抗性,利用Williams鉴别寄主系统鉴定了分离自安徽等6个省病区的16个根肿病菌菌株的生理小种(致病型),并利用温室人工接种技术鉴定评价了12份油菜抗性材料的差异、病圃鉴定了176份油菜品种资源的抗性。结果表明,来自安徽(休宁、黟县、广德)、湖北(沙阳、当阳)、四川(广汉、眉县、邛崃)和贵州金沙的油菜根肿病菌菌株为4号小种,安徽宁国、云南楚雄、湖南桃江、辽宁沈阳和黑龙江阿城的菌株为2号小种,安徽绩溪的菌株为5号小种,湖北黄陂的菌株为7号小种。在温室人工接种条件下,12份抗性资源对2号、4号和5号小种等表现出不同程度的抗性,其中抗性材料CR5对来自安徽休宁、黟县、广德和四川广汉的4号小种、安徽宁国2号小种和安徽绩溪5号小种均表现出完全的免疫抗性。田间病圃鉴定结果表明种都油998和蓉油9号两个品种表现抗病(R),富油杂118等11个品种表现中抗(MR)。

Clubroot disease caused by Plasmodiophora brassicae became serious in winter rapeseed production in China. To identify pathotypes of different isolates and to evaluate resistance of rapeseed resources,16 isolates of P. brassicae from 8 provinces were identified with Williams differential hosts in green house. Results indicated 9 isolates from Anhui（Xiuning,Yixian and Guangde）,Sichuan（Guanghan,Meixian and Qionglai）,Hubei（Shayang,Dangyang） and Guizhou（Jinsha） belonged to pathotype 4. The other 4 isolates from Anhui（Ningguo）,Yunnan（Chuxiong）,Hunan（Taojiang）,Liaoning（Shenyang） and Heilongjiang（Aching） belonged to pathotype2. Isolates from Anhui（Jixi） belonged to pathotype 5 and those from Hubei（Huangpi） belonged to pathotype 7.In artificial climate chamber,12 resistant lines were respectively inoculated by 7 isolates. Results showed that all12 resistant lines had different resistant degree to 3 pathotypes（2,4 and 5）. Resistant line CR5 was immune to 6isolates from Anhui and Sichuan provinces. In field disease nursery（using pathotype 4）,176 rapeseed cultivars were evaluated. Among them,Zhongdouyou 998 and Rongyou 9 had resistance（R）,and 11 cultivars had moderate resistance（MR） to pathotype 4.