实时荧光定量PCR检测CD44v8在膀胱及尿路上皮癌中的表达

The detection of the expression of CD44v8 with RT-PCR in bladder and urothelial carcinoma

目的探讨CD44v8在膀胱及尿路上皮癌的临床诊断价值。方法收集实验组患者膀胱及尿路上皮癌患者75例,采用实时荧光定量PCR（RT-PCR）从病理和临床分期2个方面对CD44v8进行定量分析。对照组20例患者为正常膀胱黏膜组织。结果 CD44v8蛋白在对照组膀胱黏膜上皮无表达,拷贝数小于1×10^2拷贝/毫升。75例膀胱及尿路上皮癌患者阳性表达26例（34.7%）,CT值均小于35,基因拷贝数均大于1×10^4拷贝/毫升。阳性表达率与肿瘤病理分级、肿瘤分期（TNM）相关,与肿瘤复发和数量无相关性。结论 CD44v8可作为判断膀胱及尿路上皮癌分级、分期的参考指标,具有临床诊断意义。

Objective To explore the expression of CD44v8 protein in human bladder and urothelial carcinoma,as well as the value in diagnosis of human bladder and urothelial carcinoma.Methods RT-PCR was used to analysis the expression of CD44v8 protein in 75 patients with bladder and urothelial carcinoma in the pathological stage and clinical stage and 20 subjects of normal bladder mucosa were collected as control.Results CD44v8 protein was negative in all normal bladder and urothelial mucosa,the copy number was less than 1×10^2 copy/mL;26cases of bladder and urothelial carcinoma was positive,and the positive rate of CD44v8 was 34.7%,and Ct values were less than 35 and copy number was greater than 1×10^4 copy/mL.Positive rate was correlated with high pathological grades and TNM stages,but no significant difference was observed in recurrence of tumor.ConclusionCD44v8 could be useful indicator for the assessment of pathological grades and TNM stages of bladder and urothelial carcinoma.