射频消融建立兔急性肝损伤模型

Establishment of acute liver injury model in rabbits via radiofrequency ablation

【目的】利用射频消融建立急性肝损伤模型,探讨一种稳定、高效的建模方法。【方法】16只新西兰兔随机分为4组用于观察不同时长射频后新西兰兔存活时间,以确定射频消融建立模型最佳时长;另16只新西兰兔随机分为实验组和对照组,使用最佳时长作用于实验组动物肝脏右后叶,分别在0、6 h后观察血清谷丙转氨酶（alanine aminotransferase,ALT）、谷草转氨酶（aspartate aminotransferase,AST）活性及肝组织病理学的变化,考察肝损伤模型一致性、稳定性、可靠性。【结果】通过观察新西兰兔存活时间,确定射频作用最佳时间为6~9 min;射频消融后肉眼观察损伤部位与周围组织界限清楚,损伤范围一致;病理学检查可见肝实质大面积坏死、细胞核崩解、肝血管严重出血;对照组和实验组动物血清ALT分别为（70.3±28.6）、（323.4±30.8）IU/L（P〈0.05）,血清AST分别为（56.9±38.2）、（442.5±50.4）IU/L（P〈0.05）。【结论】射频消融可以高效、稳定的建立可复制的急性肝损伤模型,为进一步探索肝损伤的机制及治疗提供有效的动物模型。

【Objective】To establish a model of acute liver injury via radiofrequency ablation（RFA）,and explore a stable and efficient modeling method.【Methods】Firstly, 16 New Zealand rabbits were randomly divided to 4 groups. Their survival time after different RFA duration was observed to determine the optimal time for the model via RFA. Then another 16 New Zealand rabbits were randomly divided into experimental group and control group. RFA in the optimal time was performed in the right posterior lobe of experimentalgroup animal liver, Serum activities of alanine aminotransferase（ALT） and aspartate aminotransferase（AST） as well as pathological changes of liver tissues at 0 h and 6 h after RFA were observed respectively to estimate the consistency, stability and reliability of liver injury model.【Results】The survival time of New Zealand rabbits determined the optimal RFA time which was 6~9 min. After RFA,the damage location and the surrounding tissue boundaries were clearly defined and the damage range was consistent by macroscopical observation. Liver parenchyma necrosis, nuclear disintegration and severe hepatic vascular hemorrhage could be observed by pathological examination. Serum ALT activities of control group and experimental group were（70.3 ± 28.6） and（323.4 ± 30.8）respectively（P0.05）, and serum AST activities were（56.9±38.2） and（442.5±50.4）（P0.05）.【Conclusion】The reproducible model of acute liver injury can be established efficiently and stably via RFA. It may be an effective animal model for further exploring the mechanism and treatment of liver injury.