摘要
OBJECTIVE: To evaluate the protective effect of puerarin [an isoflavone compound extracted from Gegen(Radix Puerariae Lobatae)] in scleral remodeling induced by extremely low frequency electromagnetic fields(ELF-EMFs).METHODS: Human fetal scleral fibroblasts(HFSFs)were divided into 5 groups:(a) untreated controls;(b) cells treated with ELF-EMFs;(c) cells treated with ELF-EMFs and puerarin 0.1 μM;(d) cells treated with ELF-EMFs and puerarin 1 μM;(e) cells treated with ELF-EMFs and puerarin 10 μM. Cell proliferation activity was measured by the cell-counting kit-8 assay. Matrix metalloproteinase-2(MMP-2) activity was measured by gelatin enzymography.MMP-2 and collagenⅠ(COL1A1) m RNA, protein expression were measured by Real-Time polymerase chain reaction, Western blot analysis, respectively.RESULTS: Puerarin reduced the inhibition in cell proliferation, MMP-2 activity, m RNA, protein expression of HFSFs exposed to ELF-EMFs and enhanced the COL1A1 m RNA and protein expression.CONCLUSION: Puerarin was found to participate in the matrix remodeling process. It might be a potential agent for the treatment of extracellular matrix degradation of sclera associated with ocular conditions.
OBJECTIVE： To evaluate the protective effect of puerarin [an isoflavone compound extracted from Gegen（Radix Puerariae Lobatae）] in scleral remodeling induced by extremely low frequency electromagnetic fields（ELF-EMFs）.METHODS： Human fetal scleral fibroblasts（HFSFs）were divided into 5 groups：（a） untreated controls;（b） cells treated with ELF-EMFs;（c） cells treated with ELF-EMFs and puerarin 0.1 μM;（d） cells treated with ELF-EMFs and puerarin 1 μM;（e） cells treated with ELF-EMFs and puerarin 10 μM. Cell proliferation activity was measured by the cell-counting kit-8 assay. Matrix metalloproteinase-2（MMP-2） activity was measured by gelatin enzymography.MMP-2 and collagenⅠ（COL1A1） m RNA, protein expression were measured by Real-Time polymerase chain reaction, Western blot analysis, respectively.RESULTS： Puerarin reduced the inhibition in cell proliferation, MMP-2 activity, m RNA, protein expression of HFSFs exposed to ELF-EMFs and enhanced the COL1A1 m RNA and protein expression.CONCLUSION： Puerarin was found to participate in the matrix remodeling process. It might be a potential agent for the treatment of extracellular matrix degradation of sclera associated with ocular conditions.
基金
Supported by the General Program of the Bio-medical Division of the Shanghai Science and Technology Commission:Effect of Extremely Low Frequency Electromagnetic Fields on Myopia Based on RPE-sclera Complex(No.10411966200)
the Scientific Research Fund of Chinese Medical of Shanghai Health Bureau:Effect of Puerarin on RPE Cells Exposed to ELF-EMF(No.2014JP015)
