摘要
The pleiotropic drug resistance（PDR） sub-family of adenosine triphosphate（ATP）-binding cassette（ABC） transporter had been reported to participate in diverse biological processes of plant. In this study, we cloned three novel PDR genes in Fusarium head blight（FHB） resistant wheat cultivar Ning 7840, which were located on wheat chromosomes 6A, 6B and 6D. In phylogeny, these genes were members of cluster I together with Ae PDR7 and Bd PDR7. Subcellular localization analysis showed that Ta PDR7 was expressed on the plasmalemma. The quantitative real time PCR（RT-PCR） analysis showed that this gene and its probable orthologues in chromosomes 6B and 6D were both up-regulated sharply at 48 h after infected by Fusarium graminearum and trichothecene deoxynivalenol（DON） in spike. When knocking down the transcripts of all Ta PDR7 members by barely stripe mosaic virus-induced gene silencing（BSMV-VIGS） system, it could promote the F. graminearum hyphae growth and made larger pathogen inoculation points in Ning 7840, which suggested that Ta PDR7 might play an important role in response to F. graminearum. Although salicylic acid（SA）, methyl jasmonate（Me JA） and abscisic acid（ABA） had been reported to possibly regulate wheat FHB resistance, here, we found that the three members of Ta PDR7 were negatively regulated by these three hormones but positively regulated by indoleacetic acid（IAA）.
The pleiotropic drug resistance（PDR） sub-family of adenosine triphosphate（ATP）-binding cassette（ABC） transporter had been reported to participate in diverse biological processes of plant. In this study, we cloned three novel PDR genes in Fusarium head blight（FHB） resistant wheat cultivar Ning 7840, which were located on wheat chromosomes 6A, 6B and 6D. In phylogeny, these genes were members of cluster I together with Ae PDR7 and Bd PDR7. Subcellular localization analysis showed that Ta PDR7 was expressed on the plasmalemma. The quantitative real time PCR（RT-PCR） analysis showed that this gene and its probable orthologues in chromosomes 6B and 6D were both up-regulated sharply at 48 h after infected by Fusarium graminearum and trichothecene deoxynivalenol（DON） in spike. When knocking down the transcripts of all Ta PDR7 members by barely stripe mosaic virus-induced gene silencing（BSMV-VIGS） system, it could promote the F. graminearum hyphae growth and made larger pathogen inoculation points in Ning 7840, which suggested that Ta PDR7 might play an important role in response to F. graminearum. Although salicylic acid（SA）, methyl jasmonate（Me JA） and abscisic acid（ABA） had been reported to possibly regulate wheat FHB resistance, here, we found that the three members of Ta PDR7 were negatively regulated by these three hormones but positively regulated by indoleacetic acid（IAA）.
基金
supported by the National Natural Science Foundation of China (3315203911, 31471488 and 31171553)
the National High-Tech R&D Program of China (2011AA100102 and 2014CB138100)
the Transgenic Special Item, China (2016ZX08009-003 and 2016ZX08002003002)
the Natural Science Foundation of Guizhou Province, China (Qianke 2015-2116)
the Scientific Research Starting Fund of Doctor of Guizhou Normal University, China
Open Subject of National Key Laboratory of Crop Biology, China (2015KF05)
