SGK1调节Th17细胞分化在高血压中的作用

The function of SGK1 in hypertension by regulating Th17 differentiation

目的探讨SGK1在高血压外周血CD4^+T淋巴细胞中的表达,揭示SGK1通过调节Th17细胞的功能在高血压中的作用。方法收集高血压患者病例,依据临床诊断分成I级、II级和III级。FCM检测Th17细胞在高血压患者外周血中的比例,ELISA检测IL-17A在血清中的表达水平。从高血压患者外周血单个核细胞中分选CD4^+T淋巴细胞,real-time PCR检测SGK1的表达,同时检测RORC的表达水平。构建高血压大鼠模型,HE染色观察肾组织炎症变化,免疫组织化学检测肾组织中SGK1的水平。结果与健康对照相比,高血压患者外周血Th17细胞的比例和血清中IL-17A的水平明显升高,且随高血压分级越高,Th17细胞比例和IL-17A血清水平越高。SGK1的表达在高血压患者CD4^+T淋巴细胞中显著升高。更为重要的是,SGK1的表达与RORC和IL-17A具有正相关性。在高盐诱导的高血压大鼠模型中,血清IL-17A的水平明显高于正常对照组。与对照组比较,高血压组的肾组织出现了明显的炎症变化和浸润的淋巴细胞,SGK1在肾组织中的表达明显升高。结论高血压中Th17细胞的比例明显升高,SGK1通过调节Th17细胞的功能促进了高血压的进展。

Objective To study the expression of SGK1 in CD4^＋ T from peripheral blood mononuclear cells（PBMC） of hypertension patients, and to explore the function of SGK1 in hypertension by regulating Th17 cells differentiation. Methods The patients with hypertension were recruited and divided into hypertension I, hypertension II and hypertension III according to clinical diagnosis of hypertension. The percentage of Th17 cells were analyzed by FCM. ELISA was using to analyze the level of IL-17 A in patients＇ serum with hypertension. The CD4^＋ T cells were prepared from PBMC of hypertension patients by using magnetic beads. The expression of SGK1 and RORC were detected by real-time PCR. The hypertension animal models were constructed by high salt diet. The inflammation form kidney was observed by HE staining, and SGK1 expression in kidney was detected by IHC. Results Compared with the healthy control, the percentage of Th17 cells and the level of IL-17 A in serum were increased significantly in patients with hypertension. Importantly, the levels were higher along with the development of hypertension. SGK1 expression was up-regulated remarkably in CD4^＋ T cells from patients with hypertension. What＇s more, SGK1 expression has positive correlation with RORC and IL-17 A. In the hypertension animal models, compare with control, the levels of serum IL-17 A was increased, the inflammation were observed in hypertension animal models and the expression of SGK1 in kidney was increased significantly. Conclusion The percentage of Th17 cells were increased in patients with hypertension, and SGK1 promoted the progress of hypertension by regulating the differentiated of Th17 cells.