Id-1和MMP-9在结直肠癌中的表达及与临床病理因素的关系

Expressions and clinicopathologic characteristics of Id-1 and MMP-9 in colorectal adenocarcinoma tissues

目的:通过研究DNA结合分化抑制蛋白1(inhibitors of DNA binding-1,Id-1)和基质金属蛋白酶9(matrix metalloproteinase-9,MMP-9)在结直肠腺癌中,分析Id-1和MMP-9与结直肠腺癌进展的关系。方法 :采用免疫组织化学法、RT-PCR和Western blot检测Id-1和MMP-9在50例结直肠癌患者癌组织及50例正常组织中的表达情况,分析二者与各临床病理因素的相关性。结果:Id-1和MMP-9在结直肠癌组织中的阳性表达率分别为72.00%(36/50)和78.00%(39/50),而在正常结直肠组织中的阳性率为24.00%(12/50)和22.00%(11/50),差异均有统计学意义(P<0.01)。Id-1和MMP-9在癌组织中的表达呈正相关(r=0.422,P=0.002)。Id-1和MMP-9的表达与结直肠腺癌的浸润深度、TNM分期、肝转移、淋巴结转移和脉管浸润具有相关性(P均<0.05),而与患者的性别、年龄、肿瘤大小和分化程度无关(P均>0.05)。RT-PCR和Western blot检测结果与免疫组织化学检测结果基本一致。生存分析显示Id-1和MMP-9表达与结直肠癌患者预后关系密切,Id-1和MMP-9高表达提示预后不良。结论:Id-1和MMP-9在结直肠癌中高表达,其表达水平与结直肠腺癌的浸润、转移具有高度相关性,二者联合检测对判断结直肠癌的侵袭、转移及预后具有重要指导意义。

Objective：To explore the expressions of inhibitors of DNA binding 1（Id-1）and matrix metalloproteinase-9（MMP-9）in human colorectal adenocarcinoma tissues and study their correlation with the progression of colorectal adenocarcinoma. Methods：The expressions of Id-1 and MMP-9 in 50 specimens of coloreetal adenocarcinoma tissues and 50 specimens of normal eolorectal tissues were detected by immunohistochemistry,reverse transcription polymerase chain reaction（RT-PCR）,and Western blotting assay.Correlations between the expressions and clinicopathological features were analyzed. Results：The positive rates of Id-1 and MMP-9 in colorectal adenoeareinoma tissues were 72.00% and 78.00%,respectively,while in normal colorectal tissues were 24.00% and22.00%,respectively. The differences were significant（all P 〈0.01）. Expression of Id-1 was positively related with expression of MMP-9 in colorectal adenocarcinoma tissues（r=0.469,P=0.000）. Expression levels of Id-1 and MMP-9 were correlated with the depth of tumor invasion,TNM stage,lymph node metastasis,vessel invasion,and liver metastasis（all P 〈0.05）,but not with age,gender,tumor size,and differentiation degrees（all P 〉0.05）. The results of RT-PCR and Western blotting assay were consistent with those of immunohistochemistry. Cox multi-factor analysis showed that Id-1 and MMP-9 expressions were independent prognosis factors for colorectal adenocarcinoma. Conclusion：The expression levels of Id-1 and MMP-9 have a high correlation with the invasion and metastasis of colorectal adenocarcinoma,and joint detection of the above indicators to judge the invasion,metastasis and prognosis ofcolorectal cancer has important guiding significance.