移植肾急性排斥反应相关免疫基因芯片的应用

Application of immune gene array related to acute rejection of renal transplantation operation

目的通过基因芯片技术研究与急性排斥反应(AR)相关的差异表达基因,为提高肾移植术后AR的早期诊断水平及肾移植的疗效提供实验依据。方法以手术当日和移植肾穿刺活检当日收集的外周血作为对照血样和实验血样,提取总RNA,反转录合成c DNA,芯片杂交,筛选差异表达基因。结果发现了外周血淋巴细胞中与AR可能相关的免疫基因,其中外周血淋巴细胞免疫相关基因差异表达上调20个基因,下调11个基因。结论外周血淋巴细胞中存在与AR可能相关的免疫基因,筛选出AR时表达量出现差异的基因。

Objective To explore the deference expression of genes related with acute rejection（AR）by gene array,in order to provide experimental evidence for improve the level of early diagnosis and the efficacy of renal transplantation. Methods The peripheral blood were collected at the day of operation and kidney puncture biopsy and were served as the control sample and lab sample, total RNA were extracted,c DNA probe were synthesized by reverse transcription,array hybridization were implemented,differentially expressed genes were screened. Results The study discovered immune gene in peripheral blood lymph cell which was possibly related with AR,there were 20 up-regulated and 11 down-regulated immune associated gene differential expression in peripheral blood lymph cell. Conclusion There were immune gene with AR may be related in perioheral blood lymph cell and the immune gene whose express-dose appears difference at the AR has been filtered.