响应面法优化合萌总黄酮提取工艺

Optimization of Extraction for Flavonoids from Aeschynomene indica L. by Response Surface Methodology

目的:利用响应面法优化合萌中总黄酮的提取工艺条件。方法:通过单因素试验分别考察提取时间、乙醇体积分数、液固比、提取温度及提取次数对合萌总黄酮得率的影响,并选取提取时间、乙醇体积分数、液固比、提取温度为影响因子,应用响应面法优化提取工艺。结果:合萌总黄酮的最佳提取条件为:提取时间60 min,乙醇浓度65%,液固比(g∶m L)25∶1,提取温度90℃,在此条件下,合萌总黄酮得率为4.08 mg/g,接近模型预测值4.11 mg/g。结论:响应面法在一定程度上可以提高合萌总黄酮得率,表明响应面法用于合萌总黄酮提取工艺的优化具有可行性。

Objective： Response Surface Methodology （RSM） was used to optimize the extraction technology of total flavonoids from Aeschynomene indica L.. Methods： In single - factor tests, extraction time, ethanol concentration, liquid -solid ratio, extraction temperature and extraction times on yield of total flavonoids were explored. Among them, extraction time, ethanol concentration, liquid - solid ratio and extraction temperature were selected as influencing factors during extraction. The extraction technology was optimized by RSM. Results： The optimal extraction conditions of total flavonoids were extraction time of 60 min, ethanol concentration of 65%, liquid -solid ratio （g： mL） of 25： 1, extraction temperature of 90 ℃. Under the optimal conditions, the yield of total flavonoids was 4.08 mg/g, which was close to the predicted value （4.11 mg/g）. Conclusion： RSM can improve the yield of total flavonoids in a certain extent, which indicated that RSM is feasible to optimizing the extraction technology of total fla- vonoids from Aeschynomene indica L. .