摘要
目的探讨miR-21和miR-93的表达水平与三阴性乳腺癌（TNBC）发生发展的相关性。方法选择行乳腺癌根治术的女性患者86例,收集癌组织与正常乳腺组织,采用逆转录聚合酶链反应（RT-PCR）检测miR-21和miR-93的相对表达量,并分析其与临床病理特征的相关性。结果 miR-21和miR-93在TNBC组织中相对表达量高于癌旁组织;TNBC组织中,miR21高表达54例,占62.79%,miR-93高表达50例,占58.14%;miR-21和miR-93在肿瘤直径〉5 cm、临床分期为Ⅲ~Ⅳ期以及淋巴结转移的患者中高表达率显著增高（P〈0.01）;miR-21和miR-93的表达水平与患者年龄、月经状态、病理分级无明显相关性。癌组织中miR-21与miR-93表达水平呈显著正相关（r=0.761,P〈0.01）。结论 miR-21与miR-93在TNBC中表达异常增高,且与肿瘤直径、临床分期、淋巴结转移有关,可作为TNBC诊断及判断预后的辅助指标。
Objective To explore the correlation between expression levels of miR-21,miR-93 and the occurrence and development of triple-negative breast cancer（ TNBC）. Methods A total of 86 females with radical mastectomy were selected to collect cancerous tissues and normal mammary tissues. Reverse transcriptase polymerase chain reaction（ RT-PCR） was used to detect the relative expression of miR-21 and miR-93,and its relationship with clinical pathological characters was analyzed. Results The expression of miR-21 and miR-93 was significantly higher in TNBC tissues than that in para-canerous tissues. Of all patients,54 patients（ 62. 79%） had high expression of miR-21 and 50（ 58. 14%） cases had high expression of miR-93 in TNBC tissues. The expression of miR-21 and miR-93 increased significantly in patients with tumor diameter 〉5 cm,in clinical stage of Ⅲ ~Ⅳ and with lymph node metastasis（ P〈0. 01）. Meanwhile,the expression of miR-21 and miR-93 had no significant association with patients＇ age,menstrual status and pathological degree. However,the expression levels of miR-21 and miR-93 were positively associated with cancerous tissues（ r = 0. 761,P〈0. 01）. Conclusion The expression levels of miR-21 and miR-93 are abnormally high in TNBC tissues,and are in close association with tumor diameter,clinical stage and lymph node metastasis,so they can be used as complementary indexes for the diagnosis and prognostic evaluation in patients with TNBC.
出处
《实用临床医药杂志》
CAS
2016年第19期31-33,共3页
Journal of Clinical Medicine in Practice
基金
中国高校医学期刊临床专项资金(11525862)
