雪莲果低聚果糖组成和含量的研究

Determination of composition and content of Yacon FOS

目的比较新来源雪莲果低聚果糖和不同厂家低聚果糖的含量和组成。方法 0.3%柠檬酸溶液超声提取、阳离子聚丙烯酰胺絮凝,sevag法除蛋白,醇沉得雪莲果低聚果糖,采用HPLC-ELSD测定雪莲果低聚果糖及不同厂家低聚果糖的含量和组成。结果雪莲果低聚果糖中GF_2占24.753%、GF_3占33.970%、GF_4占17.696%;低聚果糖A中GF_2占61.12%、GF_3占29.025%、GF_4占1.574%;低聚果糖B中GF_2占54.014%、GF_3占38.535%、GF_4占1.094%。结论雪莲果低聚果糖及不同厂家低聚果糖的含量和组成的差别较大,雪莲果低聚果糖的GF_4含量明显高于其他低聚果糖。

Objective：Comparison of FOS composition and content from different manufactures. Method：Ultrasonic extraction by 0.3% citric acid solution,cationic polyacrylamide flocculation,removing protein by sevag method,yacon FOS was obtained by alcohol. The content and composition of FOS was analyzed by HPLC-ELSD. Result Yacon FOS compositon was ：GF2 24.753%,GF3 33.970%,GF4 17.696%; FOSA composition was GF2 61.12%,GF3 29.025%,GF4 1.574%; FOSB composition was GF2 54.014%,GF3 38.535%,GF4 1.094%. Conclusion ：The content and composition of Yacon FOS are different from different manufactures. The content of GF4 was signifi cantly higher in yacon FOS than other polysaccharides.