摘要
目的比较三种不同原理抗核抗体(Antinuclear antibody,ANA)检测方法的性能特点,评估其临床应用价值。方法 445例血清样本分别采用酶联免疫吸附试验(ELISA)、间接免疫荧光法(IIF)和免疫印迹法(LIA)三种方法进行检测,比较三者检验结果的一致性。结果 ELISA与IIF、EILSA与LIA、IIF与LIA结果一致性比较均一般(Kappa值分别为0.573、0.725、0.566,P<0.05)。结论 ELISA法适用于ANA样本初筛,但不能够以ELISA法完全取代IIF法或LIA法,各实验室可根据患者的病情需要选择合适的检测方法。
Objective To compare the characteristics of three assay methods of antinuclear antibody(ANA) and to evaluate their clinical use. Methods 445 sera specimens were detected by Enzyme-Linked Immunosorbent Assays(ELISA),Indirect Immunofluorescence(IIF)and Line Immumoassay(LIA),then consistency of the three methods were analyzed. Results There were some differences of the consistency of the three methods(Kappa 0.573、0.725、0.566,P〈0.05)respectively. Conclusion ELISA was suitable for the screening of the ANA specimens,which could not replace IIF or LIA completely. The assay methods should be adopted according to the disease of the patients.
出处
《中国卫生标准管理》
2016年第18期151-152,共2页
China Health Standard Management
关键词
抗核抗体
酶联免疫吸附试验
间接免疫荧光法
免疫印迹法
Antinuclear antibody
Enzyme-linked immunosorbent assays
Indirect immunofluorescence
Line immumoassay