超高效液相色谱法测定人体血清中伏立康唑药物浓度

Determination of serum Voriconazole concentration by ultra performance liquid chromatography

目的采用超高效液相色谱法测定人血清中伏立康唑（VRC）浓度,并用于临床上该药的血药浓度监测。方法以地西泮为内标,色谱柱为Agilent Poroshell 120 EC-C18（4.6×100 mm,2.7μm）,流速1 ml/min,流动相为乙腈-0.2%甲酸水溶液（45∶55）,检测波长254 nm,进样量10μl,柱温40℃。结果 VRC及地西泮的保留时间分别为2.52和4.06 min;血清中VRC线性范围为0.12-12.0μg/ml（r=0.9999）,定量下限为0.12μg/ml,日内精密度相对标准偏差（RSD）均〈1.9%,日间精密度RSD均〈2.1%,低、中、高3个浓度方法回收率为98.39%、101.16%和96.63%。结论该方法用于VRC的血药浓度检测,具有操作简便、稳定性良好,节约时间等优点。

Objective To determinate the concentration of Voriconazole in human serum by ultra performance liquid chromatography（UPLC）. Methods Chromatography was performed on Agilent Poroshell 120EC-C18（4.6 × 100 mm, 2.7 μm）, the internal standard was Diazepam. The flow rate was 1 ml/min, the mobile phase was acetonitrile-water（0.2% formic acid）（45 ： 55）, and the detection wavelength was 254 nm. Injection volume was 10 μl and the column temperature was 40℃. Results The retention time of Voriconazole and the internal standard Diazepam was 2.52 min and 4.06 min, respectively. The calibration curve was linear over the concentration range of 0.12-12.0 μg/ml（r = 0.9999） with the lower limit of quantitation（LLOQ） at 0.12 μg/ml.Intra- and inter-day RSD was below 1.9% and 2.1%, respectively. The extraction recovery rate of the low,medium and high concentrations was 98.39%, 101.16% and 96.63%, respectively. Conclusions The UPLC is a simple, highly selective and sensitive method for determining serum level of Voriconazole.