Fas、cFLIP及TGF-β_1在宫颈鳞癌组织中的表达及意义

Expressions of Fas, c FLIP and TGF-β_1 in cervical squamous cancer and their implication

目的研究凋亡相关因子(Fas)、细胞型Fas相关死亡结构域蛋白样白介素-1β转换酶抑制蛋白(cFLIP)及转化生长因子β_1(TGF-β_1)在正常宫颈组织、宫颈上皮内瘤变组织和宫颈鳞癌组织中的表达,以及与宫颈鳞癌临床病理和分期的关系,探讨3者在宫颈鳞癌发生、发展中的作用,同时也为寻找宫颈癌的诊断和治疗新靶点提供理论依据。方法采用免疫组织化学法检测59例宫颈鳞癌、45例子宫颈上皮内瘤变(CIN)和15例正常宫颈组织中cFLIP、Fas及TGF-β_1的表达,并进行相关临床病理因素分析。结果在宫颈病变由良性到恶性的发展过程中,Fas和TGF-β_1的表达水平逐渐降低(P<0.05),cFLIP的表达水平逐渐升高(P<0.05)。在宫颈鳞癌发展过程中,Fas与cFLIP表达呈负相关(P<0.05),Fas与TGF-β_1的表达呈正相关(P<0.05)。结论Fas、TGF-β_1表达下调,以及cFLIP表达上调在宫颈鳞癌的发生、发展中发挥重要作用。

Objective To study the expressions of factor associated suicide（Fas）, cellular Fas-associated death domain-like interleukin-lβ-converting enzyme（FLICE）-like inhibitory protein（cFLIP） and transforming growth factor-β（TGF-β1） in the tissues of normal cervix, cervical intraepithelial neoplasia（CIN） and cervical squamous carcinoma as well as their relationships with the clinical pathology and staging of cervical squamous carcinoma, and explore their roles in the occurrence and development of cervical squamous carcinoma, so as to provide certain theoretical evidence for seeking a new target of diagnosis and treatment of cervical squamous cancer. Methods Immunohistochemical staining was used to examine the expressions of Fas, cFLIP and TGF-β1 in different cervical tissues. Their relationships with clinicopathological factors were analyzed. Results From the normal cervical tissues, CIN to cervical squamous carcinoma, the expression levels of Fas and TGF-β1 gradually decreased（P〈0.05）, and cFLIP expression gradually increased（P〈0.05）. There was a closely negative correlation between Fas and cFLIP expressions（P〈0.05）, and a positive correlation between Fas and TGF-β1 expressions（P〈0.05） in the development of cervical squamous carcinoma. Conclusions Down-regulation of Fas and TGF-β1 expressions and up-regulation of cFLIP expression play important roles in the development of cervical squamous carcinoma.