湿法粉碎辅助溶剂提取葡萄皮渣中多酚的研究

Study on shattering extraction with solvent of phenolics from grape marc

通过正交试验,优化分析确定湿法粉碎辅助溶剂提取葡多酚的最佳工艺条件。通过单因素试验研究了粉碎时间、固液比、乙醇体积分数、提取温度和时间对葡多酚产量的影响,利用正交试验优化得出各因素的最佳条件:粉碎时间30 s、料液比1∶20(m/v)、乙醇体积分数60%、提取温度60℃、提取时间1.5 h,在该条件下葡多酚产量可达到64.26 mg GAE/g,IC_(50)(DPPH)值为59.08 mg/L。

The optimal conditions for the shattering extraction with solvent of phenolics from grape marc were investigated by orthogonal array design. The effects of grinding time, material liquid radios, ethanol concentration , extraction temperature and time on phenolics yield were discussed by single factor experiments. The optimal values of the variables were as followings： grinding time was 30 s, material liquid radio was 1 ： 20 （m/v）, volume fraction of ethanol was 60%, extraction time was 1.5 h and the temperature was 60 ℃ . Under these conditions, the highest yield of phenolics was 64.26 mg GAE/g, and the IC50 （ DPPH ） value was 59.08 mg/L.