艾纳香油质量标准初步研究

Development of Quality Standard for Blumea Balsamifera Oil

以不同批次的艾纳香油为对象,通过观察其外观性状,测定其溶解性、比旋度以及相对密度等物理特性,并采用薄层层析法(TCL)和气相(GC)指纹图谱对收集的艾纳香油成分进行定性分析,对艾纳香油的质量标准进行初步研究。结果表明:艾纳香油为澄清、棕红色的液体,有特异的芳香气,易溶于乙醇、氯仿、乙醚,几乎不溶于水,比旋度宜为-26°^-30°,相对密度宜为0.94~0.98,β-石竹烯和l-龙脑TCL斑点清晰,分离良好,GC指纹图谱得到17个共有峰,均含有有效成分β-蒎烯、芳樟醇、樟脑、l-龙脑、β-石竹烯,相似度均在0.90以上。分析检测方法简便、可靠,可为艾纳香油的质量控制提供参考依据。

Different batches of Blumea Balsamifera Oil were sampled to observe their appearant traits and determine their physical traits such as solubility, specific rotation, relative density ratio, etc. Qualitative analysis was made of the samples by using thin layer chromatography（TLC） and gas chromatography（GC） finger-print. The results showed that B. balsamifera oil was pellucid and red-brown with specific aroma. It was easily dissolved in ethanol, trichloromethane, diethyl ether, almost insoluble in water. Its specific rotation was between-26° ~30°, and the density ratio between 0.94 ~0.98. TLC spots ofβ-caryophyllene and l-borneol were clear and separated well. Seventeen common peaks were collected and all contained the effective constituents of β-pinene, linalool, camphor, l-borneol and β-caryophyllene, with their similarities being higher than 0.90. These two methods were simple and reliable and can be used for providing reference and basis for the quality control of B. balsamifera Oil.