电化学传感器在H_2O_2检测中的应用

Application of Chitosan-Carboxylic Multi-walled Carbon Nanotubes(HRP-CTS/C-MWNTs/GCE) Electrochemical Sensor in H_2O_2 Detection

将辣根过氧化酶(HRP)固定在壳聚糖(CTS)-羧基化多壁碳纳米管(C-MWNTs)复合物修饰的玻碳电极(GCE)表面,制得壳聚糖-羧基化多壁碳纳米管(HRP-CTS/C-MWNTs/GCE)电化学传感器。采用傅立叶变换红外光谱仪检测复合物包埋的HRP,发现其结构性质未发生改变;采用循环伏安法对该电极的电化学性能进行研究,结果表明:在1/15 mol·L^(-1)的PBS(pH 6.98)缓冲溶液中出现1对氧化还原峰,传感器对过氧化氢有良好的电催化还原作用。过氧化氢浓度在0.1~12 mmol·L^(-1)范围内与还原峰电流呈线性关系,相关系数(r)为0.998 6,并检测出市售医用双氧水的平均含量为2.93%。

The horseradish peroxidase （HRP） was fixed on the surface of glassy carbon electrode （GCE） modified by chitosan（ CTS）-carboxylic multi-walled carbon nanotubes（MWNTs）. The mod- ified electrode thus prepared was named abbreviately as HRP - CTS/C - MWNTs/GCE. The HRP im- bedded in the complex was characterized by fourier transform infrared spectrometry. The result indica- ted that its composition had no change. The electrochemical property of HRP - CTS/C - MWNTs/GCE was studied by cyclic voltammetry. It was found that the modified electrode exhibited an excellent electrocatalytic response toward the reduction of H2O2. In 1/15 mol· L-1 PBS（pH 6.98） buffer so- lution, a pair of redox peaks were observed, and a good linear relationship（r =0. 998 6） between values of reduction peak current and H2O2 concentration was obtained in the range of 0. 1 -12 mmol·L-1. The method was applied in the detection of hydrogen peroxide the commercially available medical hydrogen peroxide, with content of 2.93%.