硫酸镁对仿刺参幼参麻醉效果的研究

Study on Aanesthetic Effect of Magnesium on Juvenile Sea Cucumber,Apostichopus japonicus

本研究以硫酸镁作为麻醉剂,比较了在不同水温条件下硫酸镁对仿刺参幼参的麻醉效果,分析了不同浓度硫酸镁对仿刺参幼参的麻醉效果。实验水温设置11℃、13℃、15℃、17℃、19℃和21℃六个温度梯度,实验用仿刺参幼参规格分为大规格(L)40.26±1.84g、中规格(M)21.83±1.72g、小规格(S)11.03±1.63g。实验结果显示:1.在硫酸镁麻醉剂浓度相同时,仿刺参规格越大麻醉所需时间越长,复苏时间却越短;随着麻醉剂浓度增加,对仿刺参幼参麻醉时间相应缩短,但复苏时间呈现逐渐延长。不同浓度硫酸镁麻醉剂对不同规格仿刺参的麻醉效果具有显著差异(P<0.05)。2.在水温11℃~20℃范围内,随着水温的升高,对仿刺参幼参的麻醉时间从26.13±17.78min缩短到21.47±3.52min,但复苏时间从26.13±17.78min增加到71.05±12.32min,不同水温条件下硫酸镁对仿刺参幼参麻醉效果影响显著(P<0.05)。研究表明,仿刺参体重在20g以下的适宜麻醉浓度为0.1~0.25mol·L-1,体重在20g以上的适宜麻醉浓度为0.25~0.35mol·L^(-1)。

The study compared and analyzed the anesthetic effect of magnesium on different-sized （large- sized（40.26±1.84g）, medium-sized（21.83±1.72） and small-sized（11. 03±1.63g） juvenile sea cucumber （Apostichopus japonicus） at different concentration and different water temperature （11℃, 13℃, 15℃, 17℃, 19℃, 21℃ ）. The bigger sea cucumber required more time to be anesthetized and less time to recover. The increasing of the magnesium concentration could shorten the anesthesia time and prolong the recovery time. The anesthetic effect of magnesium was significantly different at different concentration for different sizedsea cucumber （P〈0.05）. Within the temperature range from 11℃ to 20℃ the anesthetic time for sea cucumber was shortened from 26.13±17.78 min to 21.47±3.52min, while the recovery time increased from 26. 13±17.78 min to 71.05±12.32 min. The anesthetic effect was significantly different at different temperature （P〈0.05）. These results suggest that the effective anesthetic concentration of magnesium is 0.1±0.25mol L-1 for individuals less than 20g in body weight and 0.25±0.35mol L-1 for individuals more than 20g in body weight.