脾虚证大鼠骨骼肌变化及中医补脾类方的作用比较

Comparison of reinforcing spleen formulas on skeletal muscle in rats with spleen deficiency pattern

目的观察脾虚证模型大鼠骨骼肌肌力变化及中医补脾类方的作用。方法雄性Wistar大鼠随机分为正常对照组和脾虚模型组,模型组采用过度疲劳+饮食失节的方法造模4周。造模2周后模型组大鼠随机分为脾虚模型组、中药干预组(健脾升阳的补中益气汤组、健脾的四君子汤组、健脾渗湿的参苓白术散组);于造模第15天,中药各组大鼠分别按4.05 g/(kg·d)给予各干预方(补中益气汤、四君子汤、参苓白术散)灌胃;模型组和对照组给予等量蒸馏水,连续14 d。测定各组大鼠的抓握力,血液和骨骼肌相关生化指标,观察骨骼肌病理变化。结果与正常组相比,脾虚模型组大鼠抓握力、骨骼肌琥珀酸脱氢酶(SDH)、肌酸激酶同工酶(CK-MM)、肌肉素(Musclin)、胆碱酯酶受体(M-AChRM2)及Ca2+-ATPase均显著性降低,血胆碱酯酶(Ach E)显著性升高(P<0.01或P<0.05);骨骼肌出现炎细胞浸润,肌纤维排列紊乱,粗细不匀,肌丝松散及肌纤维空泡样变。与模型组相比,3个干预方组大鼠的上述指标均见不同程度地改善(P<0.01或P<0.05),骨骼肌病变减轻;其中补中益气汤组的抓握力和骨骼肌SDH、CK-MM、M-AChRM2较四君子汤组和参苓白术散组显著性升高(P<0.01或P<0.05)。结论脾虚证模型大鼠存在骨骼肌的损伤,健脾、健脾渗湿、健脾升阳3方对该模型大鼠骨骼肌均有不同程度的改善作用,但以健脾升阳的补中益气汤作用最佳。

Objective To observe and compare the effects of three reinforcing spleen formulas（ Buzhong Yiqi Decoction,Sijunzi Decoction,Shenling Baizhu Powder） on the changes of skeletal muscle power in rats model with spleen deficiency pattern. Methods Male Wistar rats were randomly divided into normal group,model group,Buzhong Yiqi Decoction（ BZYQD） group,Sijunzi Decoction（ SJZD） group and Shenling Baizhu Powder（ SLBZP） group. Except the normal group,the rats of the four groups were modeled after over fatigue and improper diet for consecutive four weeks. On Day 15 after modeling,three medication groups were intragastrically administered（ i. g.） corresponding Chinese medicines at dose of4. 05 g /（ kg· d）,while model group and normal group were i. g. administered distilled water at the same dose,for consecutive 14 days. Then grip strength was measured,biochemical indexes of blood and skeletal muscle were detected,and the pathological changes of skeletal muscle were observed. Results Compared with normal group,the indexes of rats of normal group,involving grip strength,succinate dehydrogenase（ SDH） in skeletal muscle,creatine kinase isoenzyme（ CK-MM）,Ca2 ＋-ATPase,M-AChRM2 and Musclin decreased significantly,while serum Ach E increased significantly（ P〈0. 01 or P〈0.05）. Inflammatory cell infiltration was observed in skeletal muscle,and muscle fiber was scattered in disorder with uneven thickness,loose arrangement and vacuole degeneration in model group. Compared with model group,the biochemical indexes and pathological changes of the three medication groups were ameliorated,among which the grip strength,SDH,CK-MM,M-AChRM2 of skeletal muscle in BZYQD group were higher significantly than those of SJZD group and SLBZP group（ P〈0. 01 or P〈0. 05）. Conclusion Skeletal muscle injuries were found in rats with spleen deficiency pattern. In TCM,Sijunzi Decoction has been used for reinforcing spleen,Shenling Baizhu Powder been used for reinforcing spleen and eliminating dampness,and Buzhong Yiqi Decoction been for reinforcing spleen and rising yang. The three formulas could improve the skeletal muscle of rat model with spleen deficiency pattern,in which Buzhong Yiqi Decoction showed the best efficacy.