HPLC法同时测定参菊洗剂中4种成分的含量

Simultaneous Determination of Four Components in Shenju Lotion by HPLC

目的：建立同时测定参菊洗剂中苦参碱、蒙花苷、盐酸小檗碱和蛇床子素含量的方法。方法：采用高效液相色谱法。色谱柱为Dionex C_（18）,流动相为乙腈-0.1%三乙胺水溶液（用磷酸调节pH至7.2）（梯度洗脱）,流速为1.0 ml/min,检测波长为210 nm,柱温为30℃,进样量为20μl。结果：苦参碱、蒙花苷、盐酸小檗碱和蛇床子素检测进样量线性范围分别为1.400-8.400μg（r=0.999 4）、0.152-0.912μg（r=0.999 6）、0.248-1.488μg（r=0.999 9）、0.128-0.768μg（r=0.999 4）;定量限分别为84.85、3.15、15.03、7.76ng,检测限分别为24.13、0.89、4.27、2.20 ng;精密度、稳定性、重复性试验的RSD〈2%;加样回收率分别为96.29%-99.06%（RSD=1.10%,n=9）、96.49%-98.27%（RSD=0.69%,n=9）、99.26%-101.13%（RSD=0.62%,n=9）、96.27%-98.64%（RSD=0.89%,n=9）。结论：该方法操作简便、结果准确、重复性好,可用于同时测定参菊洗剂中苦参碱、蒙花苷、盐酸小檗碱和蛇床子素的含量。

OBJECTIVE：To establish a method for simultaneous determination of matrine,linarin,berberine hydrochloride and osthole in Shenju lotion. METHODS：HPLC was performed on the column of Dionex C_（18） with mobile phase of acetonitrle-0.1% triethylamine（pH was adjusted to 7.2 with phosphoric）（gradient elution）at a flow rate of 1.0 ml/min,detection wavelength was 210 nm,column temperature was 30 ℃,and injection volume was 20 μl. RESULTS：The linear range was 1.400-8.400 μg for matrine（r=0.999 4）,0.152-0.912 μ g for linarin（r=0.999 6）,0.248-1.488 μ g for berberine hydrochloride（r=0.999 9） and0.128-0.768 μg for osthole（r=0.999 4）;limit of quantitation was 84.85,3.15,15.03,7.76 ng,limit of detection was 24.13,0.89,4.27,2.20 ng;RSDs of precision,stability and reproducibility tests were lower than 2%;recoveries were 96.29%-99.06%（RSD=1.10%,n=9）,96.49%-98.27%（RSD=0.69%,n=9）,99.26%-101.13%（RSD=0.62%,n=9）and 96.27%-98.64%（RSD=0.89%,n=9）. CONCLUSIONS：The method is simple,accurate and reproducible,and can be used for the simultaneous determination of matrine,linarin,berberine hydrochloride and osthole in Shenju lotion.