紫肉甘薯药材的质量标准研究

Study on the Quality Standard of Solanum tuberdsm

目的：建立紫肉甘薯的质量标准。方法：检测药材的显微特征;采用薄层色谱法（TLC）对药材进行定性鉴别;测定药材水分、灰分、重金属和浸出物量;采用高效液相色谱法测定药材中氯化矢车菊素的含量：色谱柱为Alltima C_（18）,流动相为乙腈-0.1%三氟乙酸溶液（80∶20,V/V）,流速为1.0 ml/min,检测波长为538 nm,柱温为30℃。结果：药材粉末显微鉴别图谱清晰。紫肉甘薯的TLC图斑点清晰,分离良好。药材水分为8.5%-11.0%,总灰分为2.0%-2.9%,铅含量〈5 ppm,浸出物为15.6%-22.3%。氯化矢车菊素检测进样量线性范围为0.043-0.215μg（r=0.999 8）;精密度、稳定性、重复性试验的RSD〈2.0%;加样回收率为95.50%-99.65%（RSD=1.35%,n=9）。结论：该研究所建标准可用于紫肉甘薯的质量控制。

OBJECTIVE：To establish the quality standard for Solanum tuberdsm. METHODS：The microscopic characteristics were detected;TLC was used for qualitative identification;moisture,ash,heavy metals and the amount of extract were determined;HPLC was conducted for content determination of oxidized cyanidin：the column was Alltima C_（18）with mobile phase of acetonitrile-0.1% trifluoroacetic acid（80 ∶ 20,V/V）at a flow rate of 1.0 ml/min,detection wavelength was 538 nm,column temperature was 30℃. RESULTS：It showed clear microscopic identification map. S. tuberdsm TLC had clear spots and well separated. The water content was 8.5%-11.0%; total ash was 2.0%-2.9%; Pb was lower than 5 ppm and alcohol-soluble extracts was15.6%-22.3%. The linear range of oxidized cyanidin was 0.043-0.215 μg（r=0.999 8）;RSDs of precision,stability and reproducibility tests were lower than 2.0%;recovery was 95.50%-99.65%（RSD=1.35%,n=9）. CONCLUSIONS：The established method can be used for the quality control of S. tuberdsm.