一测多评法测定弯管列当中4种苯乙醇苷类成分

Simultaneous Quantitative Analysis of Four Phenylethanoid Glycosides in Orobanche Cernua Loefling by Multi-components with Single Marker

目的建立弯管列当中4个苯乙醇苷类成分毛蕊花糖苷、crenatoside、2’-乙酰毛蕊花糖苷、isocrenatoside的一测多评法,验证该方法在弯管列当含量测定中应用的可行性及技术适应性。方法以毛蕊花糖苷为内标,建立crenatoside、2’-乙酰毛蕊花糖苷、isocrenatoside的相对校正因子,利用相对校正因子计算3个成分的含量,实现一测多评;同时采用外标法测定这4个成分的含量,将2种方法进行分析比较。结果 24批弯管列当药材中4个苯乙醇苷成分按一测多评方法进行测定,其计算值与外标法实测值间无明显差异。结论以毛蕊花糖苷为内参物同时测定crenatoside、2’-乙酰毛蕊花糖苷、isocrenatoside的一测多评法用于弯管列当的定量分析是可行的、准确的。

OBJECTIVE To establish a method for quality evaluation and validate its feasibilities by simultaneous quantitative assay of four phenylethanoid glycosides in Orobanche cernua Loefling by multi-components with single marker. METHODS Four main phenylethanoid glycosides were selected to evaluate the quality of Orobanche cernua Loefling. Taking acteoside as internal reference substance, and the relative correlation factors of crenatoside, 2＇-acetylacteoside and isocrenatoside were determined. Their contents in 24 batches of Orobanche cernua Loefling were determined by both external standard method and quantitative analysis multi-components by single marker（QAMS）. RESULTS No significant differences were found in the quantitative results in 24 batches of Orobanche cernua Loefling determined by external standard method and QAMS. CONCLUSION It is feasible and suitable to evaluate the quality of Orobanche cernua Loefling by QAMS.